These XYPOS mice carry a transgene containing the M. musculus sex determining allele (Sry), with Y Chromosome mice exclusively developing testicular tissue.
Read More +Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
C57BL/6J mice carrying a Mus domesticus poschiavinus Y chromosome (YPOS) develop as females with ovarian tissue or as hermaphrodites with ovarian and testicular tissue. In the presence of the Tg(Sry)4Ei transgene, XYPOS mice developed exclusively testicular tissue, thus indicating that the Sry allele carried on the YPOS chromosome is responsible for development of ovarian tissue in the C57BL/6J inbred strain background.
The 129-derived 14.6-kb of genomic sequence encoding Sry, described by Gubbay et al. (Nature, 1990. v346:245), was injected into the male pronuclei of fertilized eggs obtained from mating C57BL/6J females with C57BL/6JEi-Chr YPOS hermaphrodites. One of the two transgenic male founders successfully bred and this male transmitted offspring with a low copy number insertion, designated Tg(Sry)5Ei, and offspring with a high copy number insertion, designated Tg(Sry)4Ei. This strain was initially maintained by breeding transgene-carrying Chr YPOS consomic males to C57BL/6J and subsequently to C57BL/6JEi. In 1995 embryos were generated for cryopreservation from Tg(Sry)4Ei/+ Chr YPOS males bred to C57BL/6J and C57BL/6JEi females. Cryorecovered pups will include: normal XX females; XX carrying Tg(Sry)4Ei, which will be sterile sex-reversed phenotypic males; XYPOS lacking the Tg(Sry)4Ei, which will have ovarian or ovarian and testicular tissues (hermaphrodites); and XYPOS carrying Tg(Sry)4Ei, which are normal appearing, fertile males which can be used as breeders to maintain this strain.
Expressed Gene | Sry, sex determining region of Chr Y, mouse, laboratory |
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Site of Expression | Fluorescence is detected in pre-sertoli and pre-granulosa cells of the genital ridge, expression is stronger in homozygotes. Expression is also detected in tyrosine hydroxylase-expressing neurons of the substantia nigra of the midbrain and the medial mammillary bodies of the hypothalamus in males but not females. |
Allele Name | transgene insertion 4, Eva M Eicher |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | |
Gene Symbol and Name | Tg(Sry)4Ei, transgene insertion 4, Eva M Eicher |
Gene Synonym(s) | |
Promoter | Sry, sex determining region of Chr Y, mouse, laboratory |
Expressed Gene | Sry, sex determining region of Chr Y, mouse, laboratory |
Site of Expression | Fluorescence is detected in pre-sertoli and pre-granulosa cells of the genital ridge, expression is stronger in homozygotes. Expression is also detected in tyrosine hydroxylase-expressing neurons of the substantia nigra of the midbrain and the medial mammillary bodies of the hypothalamus in males but not females. |
Strain of Origin | C57BL/6J-Chr YPOS |
Chromosome | UN |
General Note | The single founder male carried two independent multiple transgene insertions designated Tg(Sry)4Ei (with a high copy number) and Tg(Sry)5Ei (with a low copy number). Only transgenics carrying Tg(Sry)4Ei alone were used in subsequent experiments. |
Molecular Note | This transgene consists of an inbred 129 strain derived 14.6 Kb genomic fragment containing the entire Sry open reading frame and no other genes. |
When maintaining a live colony, hemizygotes may be bred to C57BL/6J inbred mice (Stock No. 000664).
When using the XYPOS mouse strain in a publication, please cite the originating article(s) and include JAX stock #002183 in your Materials and Methods section.
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