C57BL/6JEi-Tg(Sry)4Ei Chr Y^POS/EiJ

Stock number: 002183
Product name: XYPOS

Description

These XYPOS mice carry a transgene containing the M. musculus sex determining allele (Sry), with Y Chromosome mice exclusively developing testicular tissue.

Detailed description

C57BL/6J mice carrying a Mus domesticus poschiavinus Y chromosome (YPOS) develop as females with ovarian tissue or as hermaphrodites with ovarian and testicular tissue. In the presence of the Tg(Sry)4Ei transgene, XYPOS mice developed exclusively testicular tissue, thus indicating that the Sry allele carried on the YPOS chromosome is responsible for development of ovarian tissue in the C57BL/6J inbred strain background.

Development

The 129-derived 14.6-kb of genomic sequence encoding Sry, described by Gubbay et al. (Nature, 1990. v346:245), was injected into the male pronuclei of fertilized eggs obtained from mating C57BL/6J females with C57BL/6JEi-Chr Y^POS hermaphrodites. One of the two transgenic male founders successfully bred and this male transmitted offspring with a low copy number insertion, designated Tg(Sry)5Ei, and offspring with a high copy number insertion, designated Tg(Sry)4Ei. This strain was initially maintained by breeding transgene-carrying Chr Y^POS consomic males to C57BL/6J and subsequently to C57BL/6JEi. In 1995 embryos were generated for cryopreservation from Tg(Sry)4Ei/+ Chr Y^POS males bred to C57BL/6J and C57BL/6JEi females. Cryorecovered pups will include: normal XX females; XX carrying Tg(Sry)4Ei, which will be sterile sex-reversed phenotypic males; XY^POS lacking the Tg(Sry)4Ei, which will have ovarian or ovarian and testicular tissues (hermaphrodites); and XY^POS carrying Tg(Sry)4Ei, which are normal appearing, fertile males which can be used as breeders to maintain this strain.

Allele

Symbol: Tg(Sry)4Ei
Name: transgene insertion 4, Eva M Eicher
MGI accession ID: MGI:3042867
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(Sry)4Ei
Marker name: transgene insertion 4, Eva M Eicher
Chromosome: UN
Strain of origin: C57BL/6J-Chr Y^POS
Expressed genes: Sry,sex determining region of Chr Y,mouse, laboratory
Site of expression: Fluorescence is detected in pre-sertoli and pre-granulosa cells of the genital ridge, expression is stronger in homozygotes. Expression is also detected in tyrosine hydroxylase-expressing neurons of the substantia nigra of the midbrain and the medial mammillary bodies of the hypothalamus in males but not females.
Molecular note: This transgene consists of an inbred 129 strain derived 14.6 Kb genomic fragment containing the entire Sry open reading frame and no other genes.
General note: The single founder male carried two independent multiple transgene insertions designated Tg(Sry)4Ei (with a high copy number) and Tg(Sry)5Ei (with a low copy number). Only transgenics carrying Tg(Sry)4Ei alone were used in subsequent experiments.

Allele

Symbol: Sry^AKR/J
Name: sex-determining region of Chr Y- AKR/J
MGI accession ID: MGI:3575651
Generation method: Spontaneous
Marker symbol: Sry
Marker name: sex determining region of Chr Y
Chromosome: Y
Strain of origin: AKR/J
Molecular note: This allele contains all the information necessary and sufficient to initiate normal testis development but is conditionally dysfunctional on the C57BL/6 background.