C57BL/6JEi-Tg(Sry)1Ei Chr Y^AKR/J/EiJ

Stock number: 002063
Research areas: Developmental Biology Research, Reproductive Biology Research, Research Tools

Description

In this strain, XX mice that carry the Tg(Sry)1Ei transgene are sex-reversed males.

Detailed description

This is one of three transgenic strains carrying the Tg(Sry) transgene (the Y-linked Sry gene derived from a 129 inbred strain) on a C57BL/6 background with an AKR Y chromosome. All XX mice are sex-reversed males. (See also Stock No. 002708) and Stock No. 002837).

XY^AKR/J mice heterozygous for T^Orl on a C57BL/6J background develop as sex reversed females, but in the presence of the Tg(Sry)1Ei transgene these XY mice develop as normal males with testes.

Development

The 129-derived 14.6-kb of genomic sequence encoding Sry, described by Gubbay et al. (Nature, 1990. v346:245), was injected into the male pronuclei of fertilized eggs obtained from mating C57BL/6JEi females with C57BL/6JEi-Chr Y^AKR/J males. Three XY transgenic male founders were obtained and each generated a line that was maintained by backcrossing transgenic males, obligatorily carrying the consomic Chr Y^AKR/J, to C57BL/6JEi females (Eicher et al. 2001). All three strains were independently cryopreserved. The cryo-recovered males have the Chr Y^AKR/J and those that additionally carry Tg(Sry)1Ei should be used as the breeders to maintain the line. The cryo-recovered mice with two X Chromsosomes will be either lack Tg(Sry)1Ei and be wild-type or carry Tg(Sry)1Ei and be sex reversed sterile phenotypic males.

Allele

Symbol: Tg(Sry)1Ei
Name: transgene insertion 1, Eva M Eicher
MGI accession ID: MGI:3041981
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(Sry)1Ei
Marker name: transgene insertion 1, Eva M Eicher
Chromosome: UN
Strain of origin: C57BL/6JEi-Chr Y^AKR
Expressed genes: Sry,sex determining region of Chr Y,mouse, laboratory
Molecular note: This transgene consists of an inbred 129 strain derived 14.6 Kb genomic fragment containing the entire Sry open reading frame and no other genes.
General note: Three transgenic lines (referred to as TgN(Sry-129)1Ei, TgN(Sry-129)2Ei, and TgN(Sry-129)3Ei were generated which all produced sex-reversed XX transgenic male offspring. No copy number or insertion location information was provided.

Allele

Symbol: Sry^AKR/J
Name: sex-determining region of Chr Y- AKR/J
MGI accession ID: MGI:3575651
Generation method: Spontaneous
Marker symbol: Sry
Marker name: sex determining region of Chr Y
Chromosome: Y
Strain of origin: AKR/J
Molecular note: This allele contains all the information necessary and sufficient to initiate normal testis development but is conditionally dysfunctional on the C57BL/6 background.