Protocol 2571: End Point Analysis Assay - Grid2<ho-5J> Kaspar End Point

C57BL/6J-Grid2^ho-5J/J

Stock No: 000527

Version 1.1

Notes

The genotyping protocol(s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX). To genotype animals, JAX recommends researchers validate the assay independently upon receipt of animals into their facility. Reaction cycling temperature and times may require additional optimization based on the specific genotyping reagents used.

Expected Results

JAX Protocol

Protocol Primers

Primer	5' Label	Sequence 5' → 3'	3' Label	Primer Type	Reaction	Note

Reaction A

Component	Final Concentration
Reaction Mix	0.98 X
Assay Mix
MgCl2	0.39 mM
DNA

Cycling

Step	Temp °C	Time	Note
1	94.0	--
2	94.0	--
3	57.0	--
4	72.0	--	repeat steps 2-4 for 20 cycles
5	94.0	--
6	57.0	--
7	72.0	--	repeat steps 5-7 for 20 cycles

JAX uses a very high speed Taq (~1000 bp/sec), use cycling times recommended for your reagents.

Reaction B

Component	Final Concentration
ddH2O

Strains Using This Protocol

This is the only strain that uses this protocol.