C57BL/6J-Grid2^ho-5J/J

Stock number: 000527
Product name: hotfoot 5 Jackson
Research areas: Neurobiology Research

Detailed description

The glutamate receptor delta2 (Grid2) gene is predominantly expressed in the postsynaptic densities of parallel fiber-Purkinje cell synapses and plays a crucial role in cerebellar function. Many mutations have been found for this gene, and while several hotfoot spontaneous mutations involve deletions of one or more exons in the Grid2 gene, the Grid2^ho-5J hotfoot mutation has been shown to be a point mutation.

Development

Embryos produced by crossing heterozygous Grid2^ho-5J males to C57BL/6J females were cryopreserved in 1982.

Allele

Symbol: Grid2^ho-5J
Name: hotfoot 5 Jackson
MGI accession ID: MGI:1856051
Generation method: Spontaneous
Marker symbol: Grid2
Marker name: glutamate receptor, ionotropic, delta 2
Marker synonyms: B230104L07Rik; GluD2; GluRdelta2; SCAR18; tpr
Chromosome: 6
Strain of origin: C57BL/6J
Molecular note: RT-PCR revealed a single nucleotide deletion at position 1999 in exon 12 near the beginning of the region encoding TM3. This causes a frame shift leading to a stop codon in the middle of TM3.