C57BL/6J DIO Control

Stock number: 380056
Product name: Black 6 DIO Control
Strain synonyms: B6 DIO Control, B6J DIO Control, DIO Control Mouse, Diet-induced Obesity Control
Research areas: Cardiovascular Research, Diabetes and Obesity Research, Hematological Research, Metabolism Research, Mouse/Human Gene Homologs, Neurobiology Research, Research Tools, Sensorineural Research

Description

C57BL/6J DIO Control mice are provided a low fat diet with the same protein content as the diet provided to C57BL/6J DIO (diet-induced obese) mice. DIO controls do not develop the pre-type 2 diabetes phenotypes observed in DIO mice. Male C57BL/6J mice are placed on low fat diet at 6 weeks of age and study-ready animals are available between 7 and 20 weeks of age. For projects requiring a custom diet or a different strain, our project managers can work with you to get the mice you need.

Detailed description

In comparison to DIO mice fed a high fat diet, C57BL/6J mice fed a low-fat control diet do not become obese, maintain normal blood glucose and serum lipid levels, and have improved glucose tolerance (see detailed phenotypic data). They serve as a control for DIO C57BL/6J mice.

Development

The C57BL/6J inbred strain was created by Dr. CC Little from the mating of female 57 with male 52 from Miss Abbie Lathrop's stock. The same cross gave rise to the C57L and C57BR strains.

Allele

Symbol: Micrlⁿ
Name: non-responder
MGI accession ID: MGI:5014256
Generation method: QTL
Marker symbol: Micrl
Marker name: microwave induced increase in complement receptor B cells
Chromosome: 5
Strain of origin: multiple strains
General note: The following inbred strains are homozygous for the recessive QTL, Micrl, and do not respond to microwave exposure by increasing splenic C3 receptor-bearing B cells: A/J, BALB/cJ, C3HeB/FeN, C57BL/6J, C57BL/10J (inferred from the response of two congenic lines "B10.BR", "B10.D2", "B10.D2"), C58/J, AK.B6-H2^b, and B6.AK-H2^k. These recombinant inbred strains are also non-responding: BXH2/Ty, BXH6/Ty, BXH7/Ty, BXH12/Ty, and BXH14/Ty.

Allele

Symbol: Cdh23^ahl
Name: age related hearing loss 1
MGI accession ID: MGI:3028349
Generation method: Spontaneous
Marker symbol: Cdh23
Marker name: cadherin 23 (otocadherin)
Chromosome: 10
Strain of origin: multiple strains
Molecular note: Genetic complementation tests have shown allelism between the mdfw (modifier of deaf waddler) locus and the ahl locus. Further analysis has shown this is caused by a G to A transition at coding nucleotide position 753 of Cdh23 (SNP rs257098870). This hypomorphic allele changes splice donor site G-GT to A-GT, causing frame skipping of exon 7. This is predicted to delete part of the 2nd and 3rd ectodomains and cause reduced message stability. Twenty-seven strains classified with ahl and carrying the 753A allele include: CD-1, RBF/DnJ, PL/J, AKR/J, RF/J, BALB/cBy, A/WySnJ, P/J, SENCARA/PtJ, DBA/1J, ALS/LtJ, C58/J, C57BLKS/J, 129P1/ReJ, C57BR/cd, SKH2/J, BUB/Bn, MA/MyJ, LP/J, 129X1/SvJ, NOR/LtJ, A/J, C57BL/6, NOD/LtJ, DBA/2J, ALR/LtJ, C57L/J. Strains classified with ahl that DO NOT carry this mutation include: 129S1/SvImJ, C3H/HeSnJ, I/LnJ, YBR/Ei, MRL/MpJ.

Allele

Symbol: Gluchos2^C57BL/6J
Name: C57BL/6J
MGI accession ID: MGI:3626008
Generation method: QTL
Marker symbol: Gluchos2
Marker name: glucose homeostasis QTL 2
Chromosome: 11
Strain of origin: C57BL/6J

Allele

Symbol: Gluchos1^C57BL/6J
Name: C57BL/6J
MGI accession ID: MGI:3626006
Generation method: QTL
Marker symbol: Gluchos1
Marker name: glucose homeostasis QTL 1
Chromosome: 13
Strain of origin: C57BL/6J

Allele

Symbol: Gluchos3^C57BL/6J
Name: C57BL/6J
MGI accession ID: MGI:3626010
Generation method: QTL
Marker symbol: Gluchos3
Marker name: glucose homeostasis QTL 3
Chromosome: 9
Strain of origin: C57BL/6J
Molecular note: This allele confers 50% decreased plasma insulin at T30 during the intraperitoneal glucose tolerance test compared to C3H/HeH.

Allele

Symbol: P2rx7^rs48804829-T
Name: rs48804829 SNP allele with the T variant
MGI accession ID: MGI:3042190
Generation method: Spontaneous
Marker symbol: P2rx7
Marker name: purinergic receptor P2X, ligand-gated ion channel, 7
Chromosome: 5
Strain of origin: various
Molecular note: The T variant of SNP rs48804829 at coding nucleotide 1352 codes for a leucine at codon 451 which yields a peptide with severely reduced activity. This variant is found in AKR/J, C3H/HeJ, C57BL/6, C57BL/10, CBA/J, DBA/1, DBA/2, FVB/NJ, and NZO/HILtJ. The wild-type C variant, coding for proline, is found in 129P2/OlaHsd, 129S1/SvImJ, 129S5SvEvBrd, A/J, BALB/c, CAST/EiJ ,NZW, LP/J, NOD/ShiLtJ, PWK/PhJ, WSB/EiJ, Mus caroli, M. spretus, M. musculus, and M. poschiavinus. The mutation lies within a C-terminal cytoplasmic domain homologous with the TNFR 1-death domain and with an SH3 binding protein.

Allele

Symbol: Fbrwt2^C57BL/6J
Name: C57BL/6J
MGI accession ID: MGI:3834644
Generation method: QTL
Marker symbol: Fbrwt2
Marker name: forebrain weight 2
Chromosome: 11
Strain of origin: C57BL/6J
Molecular note: This allele confers increased forebrain weight compared to DBA/2J.

Allele

Symbol: Fbrwt1^C57BL/6J
Name: C57BL/6J
MGI accession ID: MGI:3834642
Generation method: QTL
Marker symbol: Fbrwt1
Marker name: forebrain weight 1
Chromosome: 1
Strain of origin: C57BL/6J
Molecular note: This allele confers increased forebrain weight compared to DBA/2J.

Allele

Symbol: Ahr^b-1
Name: b-1 variant
MGI accession ID: MGI:2667308
Generation method: Not Applicable
Marker symbol: Ahr
Marker name: aryl-hydrocarbon receptor
Chromosome: 12
Strain of origin: C57BL/6J
Molecular note: This allele encodes a high affinity, relatively heat stabile, 95 kDa receptor. PCR sequencing of cDNA revealed ten nucleotide differences between the coding sequences of the DBA/2J and C57BL/6J receptors. Five of the ten differences would cause amino acid changes. One of these, a C to T transition in exon 11 would change the arginine codon in the DBA/2J allele to an opal termination codon in the C57BL/6J allele. This change would prevent the 43 amino acid extension of mRNA translation predicted for the DBA/2J allele and account for the smaller size of the peptide produced by this allele (95 kDa vs 104 kDa for the DBA/2J allele). A second C to T transition changes a proline codon in the DBA/2J allele to leucine codon in the C57BL/6J allele, and would likely change secondary structure of the peptide and thus ligand affinity.
General note: C57BL/6 carries the responsive Ahr^b allele; DBA/2 carries nonresponsive Ahr^d. Heterozygotes (Ahr^b/Ahr^d) are responsive (J:5282). Later work identified a second (J:8895) and later a third (J:22144) allele conferring response. Thus the allele in C57, C58, and MA/My strains is now Ahr^b-1; Ahr^b-2 is carried by BALB/cBy, A, and C3H; and Ahr^b-3 by Mus spretus, M. caroli, and MOLF/Ei. The nonresponsive strains AKR, DBA/2, and 129 carry Ahrd (J:22144). Nucleotide and amino acid sequence differences between Ahr^b-1 and Ahr^d have been determined (J:17460).

Strain of origin - this allele was found in C57BL/6, C58/J, C57BR, MA/My strains

Allele

Symbol: Nnt^C57BL/6J
Name: C57BL/6J
MGI accession ID: MGI:3626282
Generation method: Spontaneous
Attributes: Null/Knockout
Marker symbol: Nnt
Marker name: nicotinamide nucleotide transhydrogenase
Chromosome: 13
Strain of origin: C57BL/6J
Molecular note: This allele contains a stretch of 17,814 bp missing between exons 6 and 12. RT-PCR demonstrated cDNA corresponding to exons 7-11 was absent. Mature protein was not detected in these mutants.

Allele

Symbol: n-TRtct5^m1J
Name: mutation 1, Jackson
MGI accession ID: MGI:5575489
Generation method: Spontaneous
Attributes: Not Specified
Marker symbol: n-TRtct5
Marker name: nuclear encoded tRNA arginine 5 (anticodon TCT)
Chromosome: 1
Strain of origin: C57BL/6J
Molecular note: This C-to-T transition, the T variant of SNP rs46447118, found in C57BL/6J and C57BL/6JEiJ but not any of the other 18 inbred strains tested, is in nucleotide 50 in the stem of the T loop and impairs maturation, leading to accumulation of a 105 nucleotide pre-tRNA band with leader and trailer sequences instead of the usual 115 nucleotide pre-tRNA, and also diminishes aminoacylation of this isodecoder.

Allele

Symbol: Nlrp12^C57BL/6J
Name: C57BL/6J
MGI accession ID: MGI:5817983
Generation method: Spontaneous
Attributes: Not Applicable
Marker symbol: Nlrp12
Marker name: NLR family, pyrin domain containing 12
Chromosome: 7
Strain of origin: C57BL/6J
Molecular note: A sequence variant is identified in C57BL/6J (T) as compared to C57BL/6N (C) at position 3,222,537 (GRCm38). The polymorphism is located in the C-terminal leucine-rich repeat domain and results in an arginine to lysine change at amino acid residue 1034 (p.R1034K).

Allele

Symbol: Apobec3^Rfv3-r
Name: recovery from Friend virus 3, resistant
MGI accession ID: MGI:3809767
Generation method: Not Applicable
Attributes: Not Applicable
Marker symbol: Apobec3
Marker name: apolipoprotein B mRNA editing enzyme, catalytic polypeptide 3
Chromosome: 15
Strain of origin: multiple strains
Molecular note: This gene locus controls recovery from viremia and leukemia induced by Friend virus (FV). This dominant allele is found in C57BL/6J, C57BL/10, and 129P2/OlaHsd strains and allows for recovery from both viremia and leukemia in H2^b mice. Recovery from viremia only is seen in H2^a mice. This allele produces a full length mRNA transcript (including exon 2) that encodes for a protein with potent anti-viral activity.

Allele

Symbol: Cd5^b
Name: b variant
MGI accession ID: MGI:6157523
Generation method: Spontaneous
Attributes: Not Applicable
Marker symbol: Cd5
Marker name: CD5 antigen
Chromosome: 19
Strain of origin: multiple strains
Molecular note: This variant is found in many inbred strains including C57BL/6J, C58/J, AKR/J, SJL/J, SWR. Sequence analysis shows that relative to the a variant of C3H/HeJ the b variant has point mutations that result in isoleucine instead of valine at amino acid 9, glutamine instead of leucine at amino acid 52, and phenylalanine instead of isoleucine at amino acid 71, all within the amino terminal scavenger receptor cysteine-rich D1 domain, in addition to 7 silent point mutations.

Allele

Symbol: Aanat^{rs216509331-A}
Name: rs216509331 SNP allele with the A variant
MGI accession ID: MGI:2180134
Generation method: Spontaneous
Attributes: Hypomorph
Marker symbol: Aanat
Marker name: arylalkylamine N-acetyltransferase
Chromosome: 11
Strain of origin: C57BL/6J
Molecular note: Low levels of melatonin production in C57BL/6J mice have been correlated with a point mutation in the gene encoding arylalkylamine N-acetyltransferase. A G to A conversion within a cryptic splice site results in the preferential incorporation of a pseudo-exon, containing an in-frame stop codon as the second codon, into the transcript. In vitro analysis demonstrates that the resulting truncated protein, which lacks several conserved C-terminal motifs, has less than 1% enzyme activity when compared to control C3H/HeJ mice.
General note: The A variant of this SNP has also been identified in strain LG/J.

Allele

Symbol: Mx1^s1
Name: myxovirus susceptibility 1
MGI accession ID: MGI:3715154
Generation method: Spontaneous
Attributes: Null/Knockout
Marker symbol: Mx1
Marker name: MX dynamin-like GTPase 1
Chromosome: 16
Strain of origin: multiple strains
Molecular note: Many inbred mouse strains have an exon 9 to 11 deletion, resulting in a null allele and susceptibility to myxoviruses, including: A/J, ABP/Le, AKR/J, AU/SsJ, BALB/cJ, BDP/J, BUB/BnJ, C3H/HeJ, C57BL/6J, C57BL/10J, C57BL/KsJ, C57L/J, C58/J, DA/HuSn, DBA/2J, FSB/GnEi, FVB/NJ, LIS/A, LP/J, MA/MyJ, MAS/A, NZB/BINJ, P/J, PL/J, RIIIS/J, RF/J, SEA/GnJ, SEC1/ReJ, SJL/J, ST/bJ, TS1/A, TW1/A. YBR/Ei, 020/A, 129/J, SF/CamEi and SK/CamEi.
General note:

The Mx genes determine resistance to the lethal effects of various myxoviruses including neurotropic avian influenza A virus injected intracerebrally, pneumotropic strains injected intranasally, and a hepatotropic strain injected intraperitoneally (J:5645, J:13136). Resistance is not dependent on presence of the thymus and is not abolished by immunosuppression or by inhibitors of macrophage function (J:5735, J:5478, J:5645). Resistance is specific for the orthomyxoviruses (J:6265). It is dependent on the presence of interferon-alpha and -beta but not -gamma (J:7365).

The resistance allele at the Mx1 locus, under induction by alpha/beta interferon, produces the 75 kDa protein MX-1, which confers resistance to the influenza virus, in the nuclei of cells carrying the allele. Susceptibility alleles do not produce the protein (J:8273). The protein is located in the nucleus (J:7703) and produces its antiviral effect by preventing synthesis of viral mRNA in the nucleus (J:7992). Nuclear localization is necessary for anti-influenza virus activity (J:1417), but mutations induced in Mx1 showed that nuclear position was not sufficient for the effect; mutations in several domains can cause its loss (J:11840). The MX-1 protein is a GTPase containing a GTP binding domain (J:1417) and this binding core is also necessary (J:21243).

Resistance is expressed by macrophages and other cells in vitro (J:6649, J:5940) but could not be transferred to susceptible animals by transfer of macrophages from resistant mice (J:6149).

Resistance to infection with two tick-borne viruses, Thogoto virus (J:8273) and Dhori virus (J:27760), is also conferred by Mx1^r.

The Mx1^r allele occurs only in strains A2G, SL/NiA, and T9, the latter being a strain derived from an influenza-resistant wild stock, and CAST/Ei, derived from Mus musculus castaneus. Most inbred strains, including C57BL/6J, C3H/HeJ, and BALB/cJ, carry an influenza susceptible Mx1^s1 allele which produces mRNA lacking exons 9, 10, and 11 of the Mx1^r allele. This large deletion apparently renders the protein incapable of providing resistance to influenza. The CBA/J, CE/J, I/LnJ, and PERA/Ei strains, also susceptible to the virus, have another form of the Mx1^s2 allele in which there is a nonsense mutation (J:9452).

Interferon is induced by viral infection and in turn induces the Mx protein (J:7703). Although some interferon-induced genes respond directly to virus invasion as well as indirectly through induction by virus-induced interferon, this primary response is very weak for the MX-1 protein in response to either influenza or Newcastle disease viruses (J:1892).

Allele

Symbol: Cox7a2l^s
Name: short
MGI accession ID: MGI:6276120
Generation method: Not Applicable
Attributes: Not Specified
Marker symbol: Cox7a2l
Marker name: cytochrome c oxidase subunit 7A2 like
Chromosome: 17
Strain of origin: multiple strains
Molecular note: This allele contains a 6 bp microdeletion causing the loss of two amino acids and truncating the protein to 111 amino acids instead of 113 amino acids. It is found in BALB/cAnCrl, BALB/cJ, C57BL/6Cr, C57BL/6JCrl, C57BL/6JOlaHsd, C57BL/6NCrl, C57BL/6NJcl, C57BL/6NHsd, C57BL/6NTac, C57BL/6NJ, B6(CG)-Tyr/J, C57BL/6JClr, C57BL/6JArc, C57BL/6JJcl, C57BL/6JJmsSlc, C57BL/6JOla, C57BL/6JOlaHsd, C57BL/6JRcc, C57BL/6JRccHsd, C57BL/6JBom, and C57BL/6JBomTac mice.
General note: Querying the sequences of the Sanger Mouse Genomes Project reveals that the short allele with its 6 bp deletion exists in C57BL/6J, C57BL/10J, C57BL/6NJ, C58/J, BALB/cJ, C3H/HeH, 129S5/SvEvBrd, NZW/LacZ, and SEA/GnJ, but the long allele lacking the deletion exists in 129S1/SvImJ, A/J, AKR/J, BTBR T⁺ Itpr3^tf/J, BUB/BnJ, C3H/HeJ, C57BR/cdJ, C57L/J, CAST/EiJ, CBA/J, DBA/1J, DBA/2J, FVB/NJ, I/LnJ, KK/HiJ, LEWES/EiJ, LP/J, MOLF/EiJ, NOD/ShiLtJ, NZB/BlNJ, NZO/HlLtJ, PWK/PhJ, RF/J, SPRET/EiJ, ST/bJ, WSB/EiJ, ZALENDE/EiJ.

Allele

Symbol: Gabra2^C57BL/6J
Name: C57BL/6J variant
MGI accession ID: MGI:6381624
Generation method: Spontaneous
Attributes: Hypomorph
Marker symbol: Gabra2
Marker name: gamma-aminobutyric acid (GABA) A receptor, subunit alpha 2
Chromosome: 5
Strain of origin: C57BL/6J
Molecular note: This is spontaneous intronic single nucleotide deletion (a shortening of the homopolymer stretch of AAAA to AAA at Chr5:71,014,639-641 (GRCm38)). It is near a spice acceptor site and causes a substantial decrease in expression at the mRNA and protein levels in homozygous brain, with additional decreases in Gabra1, Gabra3, and Gabra5 transcripts in striatum This allele can be phenoytpically reversed entirely by the correction of this intronic point deletion (see Gabra2^{C57BL/6J+em1Geh}).

Allele

Symbol: Hbb^s
Name: single
MGI accession ID: MGI:2677684
Generation method: Not Applicable
Marker symbol: Hbb
Marker name: hemoglobin beta chain complex
Chromosome: 7
Strain of origin: C57BL/6
Molecular note: This natural variant identified by protein sequencing, produces a single beta globin with one reactive cysteine (betaCyst13). Two structurally identical beta globins migrate during electrophoresis in a dimer with alpha globin as a single band, therefore the s designation.

Allele

Symbol: B2m^b
Name: b variant
MGI accession ID: MGI:6711994
Generation method: Spontaneous
Attributes: Not Applicable
Marker symbol: B2m
Marker name: beta-2 microglobulin
Chromosome: 2
Strain of origin: C57BL
Molecular note: The b variant is runs faster than the a variant on SDS PAGE and was found to have an alanine at amino acid position 85. This allele is found in the C57BL strain family including C57BL/6, C57BL/10, C57L/J, C57BR/cdJ, as well as CAST/Ei, but not in C58/J, which has the a variant.