Argonaute proteins fight viruses, plasmids and transposons, regulate gene expression, or aid DNA replication. Animals produce two distinct types of Argonaute protein: AGO and PIWI. PIWI-clade Argonaute proteins use piRNAs (which are 18-35 nucleotides long) as guides to repress transposons or other coding and noncoding mRNAs. Pirnac noncoding transcripts are expressed post-meiotically in male germ cells, and play a role in regulation of the spermatogenic transcriptome. pi9em2Pdz knock-out mice carry a 555 bp deletion (Chr9:67,733,639-67,734,194; mm10) including the promoter and the transcriptional start sites of both noncoding transcripts of the bidirectional Pirnac5 (piRNA cluster 5) gene. This strain has been used to characterize the contribution of the two non-coding RNAs to mouse fertility, and determine the targets of piRNAs produced from Pirnac5. Homozygotes are healthy and fertile. Adult homozygous males have less hyperactivated and progressively motile sperm, with no impact on fertility.

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pi9em2Pdz knock-out mice carry a 555 bp deletion including the promoter and the transcriptional start sites of both noncoding transcripts of the bidirectional Pirnac5 (piRNA cluster 5) on mouse Chromosome 9. This strain has been used to characterize the contribution of the two non-coding RNAs to mouse fertility, and determine the targets of piRNAs produced from Pirnac5.

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