B6SJL-Tg(Myh6-MYL3)L4Dsc/Mmjax

Stock number: 040772
Product name: MYL3-WT; Tg-WT-L4 ELC
Strain synonyms: Tg-WT-L4 ELC
Research areas: Cardiovascular Research, Internal/Organ Research, Research Tools

Description

MYL3-WT transgenic mice harbor human wildtype MYL3 cDNA driven by the mouse Myh6 promoter. This is a control mouse line to the MYL3*E143K (RRID:MMRRC_075946-JAX), MYL3*A57G (RRID:MMRRC_075949-JAX) and MYL3-Δ43 (RRID:MMRRC_075951-JAX) mutant transgenic lines and may be useful when studying familial cardiomyopathies.

Detailed description

The myosin, light polypeptide 3 (Myl3) gene encodes the myosin essential light chain 3 (ELC) protein, which is a component of the cardiac myosin complex and plays a critical role in cardiac muscle contraction. Mutations in this gene are associated with hypertrophic cardiomyopathy and other cardiac disorders. These MYL3-WT transgenic mice harbor human wildtype MYL3 cDNA driven by the mouse myosin, heavy polypeptide 6, cardiac muscle, alpha (Myh6) promoter.

Hemizygous mice are viable and fertile. The donating laboratory has not evaluated homozygous mice. MYL3-WT mice do not demonstrate any significant cardiac differences compared to non-transgenic littermates. These mice may be useful controls to the MYL3*E143K (RRID:MMRRC_075946-JAX), MYL3*A57G (RRID:MMRRC_075949-JAX) and MYL3-Δ43 (RRID:MMRRC_075951-JAX) mutant transgenic lines when studying familial cardiomyopathies.

Development

The MYL3-WT transgene was generated using human wildtype myosin, light polypeptide 3 (MYL3) cDNA driven by the mouse myosin, heavy polypeptide 6, cardiac muscle, alpha (Myh6) promoter. The final transgene construct included approximately 5.5kb of the mouse Myh6 promoter, including the first two exons and part of the third, followed by ~585bp cDNA of the human MYL3 and a 623bp 3' untranslated region (UTR) from the human growth hormone (hGH) transcript. The purified linearized DNA was microinjected into the pronuclei of fertilized mouse oocytes obtained from (C57BL/6XSJL)F1 matings. Founder line L4 was established and further expanded. The donating laboratory has indicated that the transgene copy number and integration site are not known. The MYL3-WT transgenic colony was maintained by crossing hemizygous mice to B6SJLF1/J mice. Upon arrival at The Jackson Laboratory, sperm was cryopreserved. To establish a live colony, an aliquot of frozen sperm was used to fertilize B6SJLF1/J oocytes.

Allele

Symbol: Tg(Myh6-MYL3)L4Dsc
Name: transgene insertion L4, Danuta Szczesna-Cordary
MGI accession ID: MGI:8242174
Generation method: Targeted
Attributes: Inserted expressed sequence; Humanized sequence
Synonyms: MYL3-WT; Tg-WT
Marker symbol: Tg(Myh6-MYL3)L4Dsc
Marker name: transgene insertion L4, Danuta Szczesna-Cordary
Marker synonyms: Tg(Myh6-MYL3)#Dsc
Chromosome: UN
Strain of origin: (C57BL/6 x SJL)F1
Molecular note: The wildtype human ventricular myosin essential light chain (MYL3) cDNA is under the control of the mouse alpha-myosin heavy chain promoter, including the first two exons and part of the third including the first two exons and part of the third, followed by the human MYL3 cDNA carrying 43bp deletion and a 630bp 3' untranslated region (UTR) from the human growth hormone (hGH) transcript. The line 4 (L4) was established and used.