B6.Cg-Calca^{tm3.1(flpo/DsRed)Rpa}/J

Stock number: 040768
Product name: Calca^FLPo:DsRed, Calca^FLPo
Research areas: Neurobiology Research, Research Tools

Description

Calca^FLPo:DsRed knock-in/knock-out mice express codon-optimized Flp recombinase (FlpO) fused to a DsRed fluorescent reporter.

Detailed description

Calca (calcitonin/calcitonin-related polypeptide, alpha) encodes a peptide hormone that is primarily expressed in the central nervous system, but can be found in numerous tissues, serving multiple functions.

Calca^FLPo:DsRed knock-in/knock-out mice express codon-optimized Flp recombinase (FlpO) fused to a DsRed fluorescent reporter. Expression of the targeted Calca gene is disrupted. These mice can be intercrossed with mice carrying FRT-flanked sequences of interest to attain their excision or treated with viral vectors, e.g., adeno-associated viruses (AAVs), to achieve FlpO-dependent expression. The mutation is expressed widely during development, but expression is restricted to cells expressing the Calca gene in adult mice. Expression of DsRed was not examined by the donating laboratory, but detection is expected to require an antibody. 

Development

A C57BL/6-derived BAC fragment was modified with a SalI restriction site introduced at the start codon of the mouse Calca gene located in exon 2. A loxP-flanked SV-Neomycin cassette was inserted just 3’ of the Sal1 site and a FLPo:DsRed cassette, containing codon-optimized Flp recombinase (FlpO) fused to Discosoma sea anemone red fluorescent protein, was inserted into the Sal1 site to create the targeting construct. The mutation was created in (129S6/SvEvTac x C57BL/6NCrl)F1-derived G4 embryonic stem (ES) cells. Resultant mice carrying the mutation were bred with Meox^Cre mice to excise the SV-Neomycin cassette. This strain was bred to C57BL/6J for more than 6 generations by the donating laboratory. Upon arrival, sperm was cryopreserved. To establish a live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

Allele

Symbol: Calca^{tm3.1(flpo/DsRed)Rpa}
Name: targeted mutation 3.1, Richard D Palmiter
MGI accession ID: MGI:7543592
Generation method: Targeted
Attributes: Recombinase-expressing; Reporter; Null/Knockout
Synonyms: Calca^FLPo:DsRed
Marker symbol: Calca
Marker name: calcitonin/calcitonin-related polypeptide, alpha
Marker synonyms: alpha CGRP; CA; Cal1; CAL6; Calc; CALC1; calcitonin; CGRP; CGRP1; CGRP-alpha; CGRP-I; Ct; Ctn; KC; PCT; RATCAL6
Chromosome: 7
Strain of origin: (129S6/SvEvTac x C57BL/6NCrl)F1
Molecular note: A targeting construct inserted the FLPo-DsRed and loxP-flanked SV-Neo into the exon 2 of the Calca gene. The loxP-flanked neo cassette was removed by cre recombinase.