Nascent polypeptide-associated complex alpha polypeptide (Naca) encodes a protein that associates with basic transcription factor 3 (BTF3) to form the nascent polypeptide-associated complex (NACA), which binds to nascent proteins emerging from ribosomes. NACA is also implicated in modification of hypoxia-inducible factor alpha (HIF-&alpha;), which is a subunit of the hypoxia-inducible factor (HIF) complex. HIF is necessary for oxygen homeostasis by regulating metabolic adaptation, erythropoiesis (red blood cell production) and angiogenesis. NACA binds to PHD2, a prolyl hydroxylase domain protein, to enable co-translational modification of HIF-&alpha;. These NacaAA mice have two amino acid substitutions (L38A and E39A) in the Naca gene in a Pro-Xaa-Leu-Glu (PXLE) motif. This mutation abolishes the interaction between NACA and PHD2. Heterozygous and homozygous mice are viable and fertile. Homozygous NacaAA mice display mild erythrocytosis. These mice may be useful for studying erythropoiesis and the HIF pathway.

The use of this mouse model is subject to the terms and conditions of the Limited Use Label License from Caribou Biosciences, Inc.

The use of this mouse strain or other product (the "Licensed Product") is subject to the terms and conditions of the Limited License from The Broad Institute.

NacaAA knock-in (KI) mice have two amino acid substitutions (L38A and E39A) in the Naca gene, resulting in modification of a PXLE motif which abolishes NACA interaction with PHD2. These mice may be useful when studying oxygen-sensing and the hypoxia-inducible factor (HIF) pathway.

This strain is hosted by NIH's MMRRC, which partners with JAX for the distribution of this and other strains. For additional information and to purchase, please visit the MMRRC site.