Nes-FlpoER mice express a tamoxifen-inducible optimized flpo/ERT2 fusion gene under the control of the rat nestin (Nes) promoter. Nestin is an intermediate filament protein expressed in the neural stem cells of developing and adult brains. In this strain, NES directs expression of the tamoxifen-inducible flpo recombinase specifically to neural progenitors in the embryo and adult neural stem cells in the developing cerebellum. When transgenic mice are bred with mice containing FRT-flanked sequences, tamoxifen-inducible flpo-mediated recombination will result in deletion of FRT-flanked sequences in the offspring. Homozygous mice are viable and fertile with no reported phenotype abnormalities. When the Nes-FlpoER transgene and the R26MASTR allele (Stock No. <a href="https://www.jax.org/strain/018903">018903</a>), which expresses an eGFPcre fusion protein upon flpo-induced deletion of a STOP cassette, are combined with a floxed gene such as Gli2 (Stock No. <a href="https://www.jax.org/strain/007926">007926</a>), recombination of the loxP sites results in mutant cells and their progeny that can subsequently be identified by the continuous expression of eGFP from the R26 allele. The Nes-FlpoER transgene is useful for mosaic mutant analysis of Nestin-expressing neural progenitors cells in the ependymal cell layer of the cerebellum after tamoxifen is administered. The donating investigator reports no recombination in the absence of tamoxifen.

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Nes-FlpoER mice express a tamoxifen-inducible optimized flpo/ERT2 fusion gene under the control of the rat Nes promoter. This strain allows temporally controlled deletion of FRT-flanked genes in neural progenitors in the embryo and adult neural stem cells in the developing cerebellum.

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