B6;129S-Senp2^tm2.1Whsu/J

Stock number: 039992
Product name: SENP2^ΔSUMOFx
Research areas: Cell Biology Research, Research Tools

Description

SENP2^ΔSUMOFx mice have loxP sites flanking exon 16 of the Senp2 gene, encoding the protease core domain. These mice may be useful when studying the role of SUMO modification during embryogenesis.

Detailed description

SENP2^ΔSUMOFx mice have loxP sites flanking exon 16 of the SUMO/sentrin specific peptidase 2 (Senp2) gene, encoding the protease core domain. Senp2 encodes an enzyme that processes newly synthesized small ubiquitin-related modifier (SUMO) into the conjugatable form and also catalyzes the deconjugation of SUMO-containing species. Deconjugation of SUMO from its substrates is essential for development of trophoblast stem cells, niches and lineages. Global deletion of SENP2 leads to midgestational lethality, and causes severe defects in the placenta which is accompanied by embryonic brain and heart abnormalities.

Homozygous mice are viable and fertile. When bred to EIIa-Cre mice (Stock No. 003724) mice, germline deletion of exon 16 creates an in frame deletion resulting in production of a SENP2^ΔSUMO truncated mutant, deficient for the SUMO protease activity. Homozygous SENP2^ΔSUMOΔ/Δ embryos are significantly smaller or underdeveloped compared to their SENP2^ΔSUMO+/+ and SENP2^ΔSUMO+/Δ littermates at E10.5. Development of all three trophoblast layers is severely impaired in the homozygous mutants. Heterozygous SENP2^ΔSUMO+/Δ mice are viable without any noticeable abnormality.

Of note, SENP2Fx mice (Stock No. 039991) have exon 4 floxed. Cre-inducible deletion of this exon creates an out of frame deletion and no protein is detected in Cre-expressing tissue.

Development

The SENP2^ΔSUMOFx targeting vector was designed to insert a loxP site upstream of exon 16 of the SUMO/sentrin specific peptidase 2 (Senp2) gene, and a second loxP site and a frt-flanked neomycin resistance (neo) cassette were inserted downstream of exon 16. The construct was electroporated into 129S6/SvEvTac-derived CSL3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the chimeric mice were bred to R26Flp mice (Stock No. 016226) to remove the neo cassette. Resulting SENP2^ΔSUMOFx offspring were bred to C57BL/6J mice (Stock No. 000664) and were further maintained on a mixed genetic background by the donating laboratory. Upon arrival at The Jackson Laboratory, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes.

Allele

Symbol: Senp2^tm2.1Whsu
Name: targeted mutation 2.1, Wei Hsu
MGI accession ID: MGI:7380302
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Synonyms: SENP2^deltaSUMOFx
Marker symbol: Senp2
Marker name: SUMO/sentrin specific peptidase 2
Marker synonyms: 2310007L05Rik; 4930538C18Rik; Axam; AXAM2; mKIAA1331; SMT3IP2
Chromosome: 16
Strain of origin: 129S6/SvEvTac
Molecular note: The targeting construct floxed exon 16. Flp-mediated recombination removed the selection cassette.