B6.FVB(C)-Tg(Pdpn-cre)HRkl/J

Stock number: 039769
Product name: B6.PDPN-Cre
Research areas: Cancer Research, Research Tools

Description

These B6.PDPN-Cre transgenic mice express cre recombinase in Pdpn-expressing lymphatic endothelial cells (LECs). When these mice are bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequences in Pdpn-expressing LECs of the offspring. These mice may be useful for studies related to the role of Pdpn-expressing cells in cancer progression and tumor metastases.

Of note, the donating investigator has also made this transgene available on a congenic BALB/c background as Stock No. 039447.

Detailed description

These B6.PDPN-Cre transgenic mice express cre recombinase under control of the mouse Pdpn (Podoplanin) gene promoter. When these transgenic mice are bred with mice containing loxP-flanked sequences, Cre-recombinase activity is detected in Pdpn-expressing lymphatic endothelial cells (LECs) of the offspring. The donating laboratory bred these PDPN-Cre transgenic mice to mice expressing a Cre-inducible tdTomato reporter allele and reports that tdTomato expression was detectable in PDPN-positive ear fibroblasts from the resulting progeny. Hemizygous B6.PDPN-Cre mice are viable and fertile. Homozygous viability/fertility has not been tested by the donating laboratory. This strain may be useful for understanding the role of PDPN positive LECs in tumor progression and metastases.

Of note, the donating investigator has also made this transgene available on a congenic BALB/c background as Stock No. 039447.

Development

The B6.PDPN-Cre transgenic construct was generated by cloning and ligating the 4kb mouse Podoplanin (Pdpn) promoter sequence to a full length cre recombinase sequence and an SV40 polyA signal using the pCR2.1-TOPO vector (Invitrogen). The construct was microinjected into FVB fertilized mouse eggs and founder line H was subsequently established. The donating investigator confirmed that a single copy of the PDPN-TK transgene integrated on chromosome 16. The mice were backcrossed to BALB/cAnNCrl for more than twenty generations by the donating laboratory (Stock No. 039447). The BALB/c congenic mice were then backcrossed to C57BL/6J, using a speed congenic protocol, for 4 generations by the donating laboratory. SNP analysis revealed that the mice were 98.1% C57BL6/J at N4. Donor mice provided to The Jackson Laboratory were at N5. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

Of note, the donating laboratory indicates that the Y chromosome was fixed during backcross to C57BL/6J.

Genotyping completed by The Jackson Laboratory (April 2025), determined that a neomycin sequence, from an unknown source, is segregating with the PDPN-Cre transgene.