STOCK Tg(HBE1-H2B/CFP)30Maba/J

Stock number: 039609
Product name: ε-globin::H2B-CFP
Research areas: Developmental Biology Research, Hematological Research, Research Tools

Description

ε-globin::H2B-CFP transgenic mice express an H2B-CFP fusion protein under the control of the human ε-globin minimal protomer and a truncated human micro-locus control region. These mice may be useful for chromatin-localized fluorescent expression in nuclei of primitive erythroid cells (EryP).

Note that the donating investigator has also made a similar transgenic line available expressing an H2B-EGFP (enhanced green fluorescent protein) fusion protein under the control of the human ε-globin minimal promoter (Stock No. 039607).

Detailed description

ε-globin::H2B-CFP transgenic mice express an H2B-CFP fusion protein (coding sequence for the human HIST1H2BB gene [histone 1 H2bb] followed C-terminally by a Cyan Fluorescent gene) under the control of the human ε-globin minimal protomer and a truncated human micro-locus control region. Hemizygous and homozygous transgenic mice are viable and fertile with no overt phenotype. Fluorescent expression is detectable in the nuclei of primitive erythroid cells (EryP) in the yolk sac (~E6.5-9.0), in the fetal livers between embryonic day E10.5-E14.5 and using flow cytometry, in the circulation throughout the rest of gestation. Fluorescence can be measured by flow cytometry and immunofluorescence. ε-globin::H2B-CFP transgenic mice are useful for chromatin-localized fluorescent expression in nuclei of primitive erythroid cells (EryP).

Note that the donating investigator has also made a similar transgenic line available expressing an H2B-EGFP (enhanced green fluorescent protein) fusion protein under the control of the human ε-globin minimal promoter (Stock No. 039607).

Development

The ε-globin::H2B-CFP transgene was designed with the human embryonic (ε) globin minimal promoter (HBE1) driving expression of an H2B-CFP fusion protein (coding sequence for the human HIST1H2BB gene [histone 1 H2bb] with a C-terminal Cyan Fluorescent Protein gene). The construct also contains a human embryonic (ε) globin 3' splice and polyA sequence and a truncated human micro Locus Control Region. The ~5.7kb transgene was microinjected into B6D2F1 fertilized oocytes, and mice from founder line number 30 were bred to outbred ICR mice to establish a colony. The transgene integration site and copy number has not been determined by the donating laboratory. Upon arrival at The Jackson Laboratory in 2024, an aliquot of frozen sperm was recovered using C57BL/6J inbred oocytes (Stock No. 000664) to establish the colony.

Allele

Symbol: Tg(HBE1-H2B/CFP)30Maba
Name: transgene insertion 30, Margaret H Baron
MGI accession ID: MGI:7703306
Generation method: Transgenic
Attributes: Reporter; Epitope tag
Synonyms: e-globin-H2B-CFP
Marker symbol: Tg(HBE1-H2B/CFP)30Maba
Marker name: transgene insertion 30, Margaret H Baron
Chromosome: UN
Strain of origin: B6D2F1
Molecular note: The transgene was designed with the human embryonic epsilon globin minimal promoter (HBE1) driving expression of an H2B-CFP fusion protein (coding sequence for the human HIST1H2BB gene [histone 1 H2bb] with a C-terminal a Cyan Fluorescent Protein gene).