The Olfr558 KO mice have loxP::frt::PGK-neo(STOP)::frt::TetO::loxP inserted just upstream of the start ATG of the Or51e1 locus. It's a knock-out (KO) first before Cre recombination. Cre recombination rescues the Or51e1 expression. Also, it can be used for Tet-controllable ectopic expression (before FLP), and after FLP, Tet-controllable Or51e1 overexpression with tTA or knockdown/KO with tTS [<a href="https://www.ncbi.nlm.nih.gov/pubmed/20163789">Tanaka et al., Biol Psychiatry 67:770</a>]. Olfactory receptor 558 (OLFR558) is one of only three mammalian ORs to have been well-conserved by evolution in mammals, including mice, rats and human. In addition to act in the olfactory epithelium (OE) to mediate the sense of smell, it is expressed in murine kidney and localizes to renin-positive cells in the kidney and to vascular smooth muscle cells where its role was largely unknown. The donating investigator's report confirms the absence of Or51e1 mRNA in the kidney, blood vessels, heart, and BAT in homozygous KO mice where Olfr558 is normally expressed. Both male and female homozygous mice are viable and fertile but show an absence of physiological blood pressure sex differences in 10-week-old mice. Female KOs exhibit increased blood pressure and increased pulse wave velocity. In contrast, male KO mice have decreased renin expression and activity, altered vascular reactivity, and decreased diastolic pressure. There were no mechanical properties differences in blood vessels between KO and WT. 
 This strain is a congenic C57BL/6J derived from the original C57BL/6N coisogenic Olfr558 KO mice (Stock No. <a href="https://www.jax.org/strain/039416">039416</a>).
 Of note, the donating investigator has donated floxed Olfr78 conditional knockout mice (Stock No. <a href="https://www.jax.org/strain/039290">039290</a>). Olfr78 is also expressed in a subset of vascular cells in renal blood vessels. This line will be useful for studies of the tissue-specific functions of Olfr558.

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Olfr558 KO mice have loxP::frt::PGK-neo(STOP)::frt::TetO::loxP inserted just upstream of the translation initiation start site of the olfactory receptor family 51 subfamily E member 1 (Or51e1; also known as Olfr558). It is a knock-out (KO) before Cre recombination. Cre recombination rescues the Or51e1 expression. Also, it can be used for Tet-controllable ectopic expression (before FLP), and after FLP, Tet-controllable Or51e1 overexpression with tTA or knockdown/KO with tTS. Olfr558 is a G protein-coupled receptor (GPCR) that is expressed in the olfactory epithelium (OE) to mediate the sense of smell. It is also expressed in the kidney cells and vascular smooth muscle to plays a role in renin secretion. This line will be useful for studies of the roles of Olfr558 in kidney and blood vessels and its involvement in blood pressure. Of note, the donating investigator has also made the Olfr558 KO available on a C57BL/6N genetic background as Stock No. <a href="https://www.jax.org/strain/039416">039416</a>).

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