129S4(Cg)-Fgfr2^tm9.1Sor/J

Stock number: 039110
Product name: Fgfr2^Flag , Fgfr2^WT-Flag
Research areas: Research Tools

Description

Fgfr2^Flag knock-in mice express fibroblast growth factor receptor 2 (FGFR2) protein with an in-frame 3xFLAG epitope tag at the 3' end. This strain is useful in labeling FGFR1:FGFR2 heterodimers in a range of studies including development, cell proliferation, differentiation, and adhesion.

Detailed description

Fibroblast Growth Factor (FGF) signaling plays an integral role in development, driving numerous cellular processes including proliferation, differentiation, and cellular adhesion.

These Fgfr2^Flag knock-in mice knock-in express fibroblast growth factor receptor 2 (FGFR2) protein with an in-frame 3xFLAG epitope tag at the 3' end. FLAG-positive staining has been observed in the epithelium of embryonic (E14.5) facial mesenchyme. This strain, in conjunction with Fgfr1^Myc mice (Stock No. 039109), is useful in detecting the presence of FGFR1:FGFR2 heterodimers in a range of research areas including immunohistochemistry, biochemical studies, and proteomics.

Development

A linearized targeting vector used to introduce an in-frame 3xFLAG epitope tag to the 3' end of the mouse Frfr2 gene was electroporated into 129S4/SvJaeSor-derived AK7 embryonic stem (ES) cells. Resultant chimeric mice were bred to Meox2-Cre deleter mice on a 129S4/SvJaeSor genetic background to remove a loxP-flanked neomycin selection cassette that had been introduced to exon 8. The Meox2-Cre allele was subsequently crossed out. Mice were maintained on a 129S4 coisogenic background by the donating laboratory.

Upon arrival at The Jackson Laboratory, sperm was cryopreserved. Cryorecovery/live colony development will involve 129S4/SvJaeSorJ (Stock No. 033000) or 129S4/SvJaeJ (Stock No. 009104).

Allele

Symbol: Fgfr2^tm9.1Sor
Name: targeted mutation 9.1, Philippe Soriano
MGI accession ID: MGI:7574142
Generation method: Targeted
Attributes: Epitope tag
Synonyms: Fgfr2^WT-FLAG
Marker symbol: Fgfr2
Marker name: fibroblast growth factor receptor 2
Marker synonyms: BBDS; BEK; BFR-1; CD332; CEK3; CFD1; ECT1; Fgfr-2; Fgfr2b; Fgfr7; Fgfr-7; JWS; KGFR; KGFRTr; K-SAM; svs; TK14; TK25
Chromosome: 7
Strain of origin: 129S4/SvJaeSor
Molecular note: A linearized targeting vector is used to introduce an in-frame 3xFLAG epitope tag to the 3' end of the gene. Cre-mediated recombination removed the loxP-flanked neo cassette in exon 8 in the construct.