Prop1 (paired like homeodomain factor 1) encodes a transcription factor required for pituitary cell differentiation and production of growth hormone (GH), prolactin, and thyroid stimulating hormone (TSH). These Prop1neo mice have a lacZ-neomycin cassette replacing exon 1, intron 1 and part of exon 2 of the Prop1 gene, resulting in a null allele. Heterozygous mice are viable and fertile with no overt phenotype. Homozygous mice are viable and fertile, but do demonstrate dwarfism, noticeable by 2 weeks of age. The donating laboratory recommends delaying weaning of homozygous mice until ~5 weeks of age. These Prop1neo homozygous mice demonstrate phenotypes similar to the <a href="https://www.informatics.jax.org/allele/MGI:1856842">Prop1df</a> allele, including stunted growth and absence of GH, prolactin and TSH. Genetic background affects the degree of respiratory distress and viability of homozygous mice with ~6.7% surviving to 2 weeks of age on the 129S1 background. After backcrossing the 129S1 homozygous mice to C57BL/6 for 4 generations, viability was increased to ~17.5% homozygous surviving to 2 weeks of age. The donating laboratory maintains these mice on a mixed B6;129S background. It is recommended that this line be maintained on a mixed B6;129 genetic background. These Prop1neo mice may be useful when studying pituitary development and hormone production.

Prop1neo has a lacZ-neomycin cassette replacing the start codon of exon 1 and part of exon 2 of the Prop1 gene. These mice may be useful when studying pituitary development and hormone production.

This strain is hosted by NIH's MMRRC, which partners with JAX for the distribution of this and other strains. For additional information and to purchase, please visit the MMRRC site.