B6;FVB-Tg(Whrn-EGFP/Rpl10)ES699Htz/J

Stock number: 037196
Product name: Whrn-bacTRAP
Research areas: Cell Biology Research, Neurobiology Research, Research Tools

Description

Whrn-bacTRAP transgenic mice express EGFP-tagged ribosomal protein L10a (Rpl10a) from the mouse Whrn (whirlin) promoter/regulatory elements. The mutation enables affinity purification of polyribosomes for analysis of ribosome‐bound mRNAs using the translating ribosome affinity purification (TRAP) method.

Detailed description

Whrn-bacTRAP transgenic mice express EGFP-tagged ribosomal protein L10a (Rpl10a) from the mouse Whrn (whirlin) promoter/regulatory elements. EGFP/Rpl10 is expressed in distinct populations of neurons throughout the brain, including the septum and lateral hypothalamus. The mutation enables affinity purification of polyribosomes for analysis of ribosome‐bound mRNAs using the translating ribosome affinity purification (TRAP) method.

The expression pattern of the transgene agrees with the Whrn in situ hybridization data available on the Allen Brain Atlas website, but fewer cells are labeled. The fluorescence of EGFP can be directly detected in the hypothalamus and inferior colliculus. Immunocytochemistry is necessary for the cortex. Images from this mouse line can be viewed on the GENSAT (Gene Expression Nervous System Atlas) website.

The integration site and copy number of the BAC transgene are unknown. Hemizygous mice are viable and do not display any obvious physical or behavioral abnormalities. The viability/fertility of homozygotes has not been assessed, and the donating laboratory does not recommend generating them due to the potential for rearrangement of the BAC concatemers and expression pattern changes.

Development

EGFP reporter fused to Rpl10a (ribosomal protein L10a) was introduced immediately upstream of the ATG start site of the mouse Whrn gene on BAC RP23-429K13. The BAC also carries sequences for the following genes, but their expression has not been confirmed: Orm2, Orm3, and Akna. The modified BAC was microinjected into the pronucleus of FVB/N zygotes and randomly integrated into the genome of the mouse. Resultant founder mice were crossed with C57BL/6J animals for 2 generations. The integration site and copy number of the BAC transgene are unknown. This strain was maintained on mixed C57BL/6J and FVB genetic background by the donating laboratory. This is founder line ES699.

Upon arrival at The Jackson Laboratory, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

Allele

Symbol: Tg(Whrn-EGFP/Rpl10)ES699Htz
Name: transgene insertion ES699, Nathaniel Heintz
MGI accession ID: MGI:7260290
Generation method: Transgenic
Attributes: Reporter
Synonyms: Whrn-bacTRAP
Marker symbol: Tg(Whrn-EGFP/Rpl10)ES699Htz
Marker name: transgene insertion ES699, Nathaniel Heintz
Chromosome: UN
Strain of origin: FVB/N
Molecular note: EGFP reporter fused to Rpl10a is introduced immediately upstream of the ATG start site of the mouse Whrn gene on BAC RP23-429K13. The BAC also carries sequences for the following genes, but their expression has not been confirmed: Orm2, Orm3, and Akna.