B6.Cg-Tg(Cdkn2a/luc/RFP/TK)1Cmps/J

Stock number: 037045
Product name: p16-3MR
Research areas: Research Tools

Description

This p16-3MR BAC transgenic strain is no longer available from The Jackson Laboratory. Please search the JAX Mice database for strains carrying any Cdkn2a mutant alleles/transgenes.

The journal where this p16-3MR BAC transgenic strain was first characterized in 2014, Developmental Cell, issued an Editorial Note in June 2026 - PMID:42412492.

Allele

Symbol: Tg(Cdkn2a/luc/RFP/TK)1Cmps
Name: transgene insertion 1, Judith Campisi
MGI accession ID: MGI:6766435
Generation method: Transgenic
Attributes: Reporter
Synonyms: p16-3MR
Marker symbol: Tg(Cdkn2a/luc/RFP/TK)1Cmps
Marker name: transgene insertion 1, Judith Campisi
Chromosome: 13
Strain of origin: C57BL/6 x 129
Molecular note: A construct consisting of a bioluminescence synthetic Renilla luciferase (hrl) reporter gene, a reporter gene encoding the monomeric red fluorescence protein (mrfp1), and a mutant herpes simplex virus type 1 sr39 thymidine kinase [HSV1-truncated sr39tk (ttk) and a PET reporter gene ("3MR") was inserted in-frame with Cdkn2a exon 2 in the mouse BAC clone RP24-322D20. Expression of this construct results a fusion protein containing the first 62 amino acids of the encoded protein and the 3MR reporters, but no full-length WT protein. Insertion of the 3MR cDNA also resulted in a stop codon in exon 2 to prevent expression from the BAC gene. This transgene is designed to identify, isolate, and selectively kill senescent cells.