STOCK Rbbp8^tm1.1Rjbr/J

Stock number: 036649
Product name: Ctip^co
Research areas: Cancer Research, Cell Biology Research, Research Tools

Description

Exon 2 of the mouse Rbbp8 (retinoblastoma binding protein 8, endonuclease; also called CtIP) gene is flanked by loxP sites in these Ctip^co conditional mutant mice (Reczek, 2016). Cre recombinase-mediated excision of the floxed region results in a defective allele that encodes an amino‐terminally truncated polypeptide, initiated at methionines M120 and/or M141 (Wang, 2020). Widespread, homozygous knock-out of the gene is embryonic lethal (Reczek, 2016), while progenitor B cell-specific inactivation of the gene (created through crosses with Cd79a^tm1(cre)Reth (Stock No. 020505) mice) impairs early B cell development (Liu, 2019).

Detailed description

Exon 2 of the mouse Rbbp8 (retinoblastoma binding protein 8, endonuclease; also called CtIP) gene, encoding the N-terminal 36 amino acids, is flanked by loxP sites in these Ctip^co conditional mutant mice (Reczek, 2016). Cre recombinase-mediated excision of the floxed region results in a defective allele that encodes an amino‐terminally truncated polypeptide, initiated at methionines M120 and/or M141 (Wang, 2020). Widespread, homozygous knock-out of the gene is embryonic lethal (Reczek, 2016), while progenitor B cell-specific inactivation of the gene (created through crosses with Cd79a^tm1(cre)Reth (Stock No. 020505) mice) impairs early B cell development (Liu, 2019). 

 

Development

A loxP site was introduced to intron 1 upstream of the transcriptional initiation site and a loxP-FRT-PGK-neo-FRT cassette was placed in intron 2 via homologous recombination in 129/Sv-derived embryonic stem (ES) cells. The FRT-PGK-neo-FRT cassette was excised through crosses with mice expressing FlpE recombinase, leaving exon 2 flanked by loxP sites (Reczek, 2016).

Allele

Symbol: Rbbp8^tm1.1Rjbr
Name: targeted mutation 1.1, Richard J Baer
MGI accession ID: MGI:6197086
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed)
Marker symbol: Rbbp8
Marker name: retinoblastoma binding protein 8, endonuclease
Chromosome: 18
Strain of origin: 129
Molecular note: A loxP site was inserted into intron 1 and a second loxP site and an FRT site flanked neomycin resistance gene cassette into intron 2. The neo cassette was removed through subsequent flp-mediated recombination, leaving a conditional-ready allele with exon 2 floxed.