Exon 3 of the mouse Pick1 gene is flanked by loxP sites in these Pick1loxP mutant mice. Cre recombinase-mediated excision of the floxed region results in a frame shift/knock-out allele that has been useful in studies of calcium-permeable AMPA receptor plasticity (CARP).
Richard L Huganir, Johns Hopkins University School of Medicine
Pick1 (protein interacting with C kinase 1) encodes a GluR2-interacting protein required for calcium-permeable AMPA receptor plasticity (CARP). Exon 3 of the mouse Pick1 gene is flanked by loxP sites in these Pick1loxPmutant mice. Cre recombinase-mediated excision of the floxed region results in a frame shift/knock-out allele. Heterozygous and homozygous floxed mice are viable and fertile.
The constitutive knock-out allele derived from this floxed allele can be found in Stock No. 008891.
A loxP site was introduced to intron 2 and a neomycin-loxP cassette was placed in intron 3 via homologous recombination in (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1 embryonic stem (ES) cells. Resultant mice were backcrossed to C57BL/6NCrl for 13 generations, then backcrossed to C57BL/6J for 4 generations by the donating laboratory.
Currently there are no related genes or alleles for this strain.
Homozygotes and heterozygotes are viable and fertile.
When using the Pick1loxP mouse strain in a publication, please cite the originating article(s) and include JAX stock #035500 in your Materials and Methods section.