The progesterone receptor (Pgr) is a nuclear steroid receptor that functions as a ligand activated transcription factor regulating gene expression following progesterone hormone binding. The progesterone receptor gene has two distinct promoters and translation start sites in the first exon which produce two isoforms, A and B. PgR_AF1_FFF knock-in mice carry K461F, K478F and R489F (lysine, lysine, arginine, respectively to phenylalanine) substitutions in the activation function 1 domain of Pgr. Males homozygous for the mutation are viable and fertile. Female PgR_AF1_FFF mice are infertile and exhibit impaired mammary and uterine development. In cell cultures the FFF mutations result in the abolition of up to 80% of progesterone receptor activity. This strain may be useful for studying the biology of the mammary gland and uterus. PgR_AF1_QQQ mice carrying the K461Q, K478Q and R489Q mutations are available as MMRRC Stock No. <a href="https://www.jax.org/strain/035417"">067159</a>.

The use of this mouse model is subject to the terms and conditions of the Limited Use Label License from Caribou Biosciences, Inc.

The use of this mouse strain or other product (the "Licensed Product") is subject to the terms and conditions of the Limited License from The Broad Institute.

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PgR_AF1_FFF knock-in mice carry K461F, K478F and R489F (lysine, lysine, arginine, respectively to phenylalanine) substitutions in the activation function 1 domain (AF1) of the Pgr (progesterone receptor) gene. Homozygous FFF females are infertile. This strain may be useful for studying the biology of the mammary gland and uterus.

This strain is hosted by NIH's MMRRC, which partners with JAX for the distribution of this and other strains. For additional information and to purchase, please visit the MMRRC site.