CByJ.B6-Tg(UBC-GFP)30Scha/J

Stock number: 034677
Product name: UBC-GFP H-2^d
Strain synonyms: UBI-GFP
Research areas: Hematological Research, Immunology, Inflammation and Autoimmunity Research, Neurobiology Research, Research Tools

Description

UBC-GFP H-2^d transgenic mice express green fluorescent protein directed by the human ubiqutin C promoter and are homozygous for the the H-2^d MHC haplotype. Certain hematopoietic cell types display distinct expression levels of GFP, allowing identification of different cells types by FACS analysis. This strain is a useful tool for studying hematopoietic cell differentiation and in vivo leukocyte tracking.

This transgene is also available on C57BL/6J as Stock No. 004353, as well as on BALB/cByJ retaining the H-2^b MHC haplotype as Stock No. 007076.

Detailed description

In 2020, Stock No. 007076 was discovered to have the UBC-GFP transgene integrated at Chr17:29,435,589 - a noncoding region centromeric from the H-2 locus (~4.6 Mbp away from H2-K1) - and retained the H-2^b MHC haplotype. Some mice were subsequently bred to BALB/cByJ (Stock No. 001026) in an attempt to generate UBC-GFP transgenic offspring with successful meiotic recombination crossover events that acquire the H-2^d MHC haplotype. By February 2022, UBC-GFP transgenic mice homozygous for H-2^d were obtained and thereafter maintained as Stock No. 034677 (UBC-GFP H-2^d).

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for Stock No. 034677. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

UBC-GFP transgenic mice express Enhanced Green Fluorescent Protein (EGFP) under the direction of the human ubiqutin C promoter.
Mice hemizygous for the transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mice express EGFP in all tissues examined. Certain hematopoietic cell types display distinct expression levels of EGFP, allowing identification of different cells types by FACS analysis. EGFP expression is uniform within a cell type lineage and remains constant throughout development. T cells have a 2-fold higher EGFP expression than CD19⁺B220⁺ B cells or peripheral blood cells. Leukocytes and red blood cells from homozygous mice fluoresce at approximately twice the level of cells from hemizygous mice. This mutant mouse strain represents a useful tool in studies related to hematopoietic cell differentiation and in vivo tracking of leukocytes.

Development

A transgenic construct containing an enhanced green fluorescent protein (EGFP) open reading frame under the control of the human ubiqutin C promoter was microinjected into fertilized C57BL/6 oocytes. Mice were screened for genomic integration of the transgene by PCR and for GFP expression in peripheral blood leukocytes. The resulting female mouse exhibiting the desired phenotype was bred to a male C57BL/6J mouse. F1 progeny from this cross that expressed GFP were intercrossed to produce homozygotes. The mice were then backcrossed to BALB/cBy for 5 generations before arriving at The Jackson Laboratory as Stock No. 007076.

In 2020, Stock No. 007076 [generation total ~N12+] was discovered to have the UBC-GFP transgene integrated at Chr17:29,435,589 - a noncoding region centromeric from the H-2 locus (~4.6 Mbp away from H2-K1) - and retained the H-2^b MHC haplotype. Some mice were subsequently bred to BALB/cByJ (Stock No. 001026) in an attempt to generate UBC-GFP transgenic offspring with successful meiotic recombination crossover events that acquire the H-2^d MHC haplotype. By February 2022 [generation total ~N14], UBC-GFP transgenic mice homozygous for H-2^d were obtained and thereafter maintained as Stock No. 034677 (UBC-GFP H-2^d).

Allele

Symbol: Tg(UBC-GFP)30Scha
Name: transgene insertion 30, Brian Schaefer
MGI accession ID: MGI:3057178
Generation method: Transgenic
Attributes: Reporter
Synonyms: Tg(UBCGFP)30Scha; UBC-GFP; Ub-GFP; UBI-EGFP; UBI-GFP; uGFP
Marker symbol: Tg(UBC-GFP)30Scha
Marker name: transgene insertion 30, Brian Schaefer
Marker synonyms: UBC-GFP; UBI-EGFP; UBI-GFP; uGFP
Chromosome: 17
Strain of origin: C57BL/6
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: Certain hematopoietic cell types display distinct expression levels of GFP. Expression is uniform within a cell type lineage and remains constant throughout development.
Molecular note: The transgene contains an enhanced green fluorescent protein open reading frame under the control of the human ubiquitin C promoter. The transgene integrated into chromosome 17 at nucleotide position 29,435,589 (reference sequence NC_000083.6; GRCm38.p6) - this is a noncoding region centromeric from the H-2 locus (~4.6 Mbp away from H-2 K1).
General note: Homozygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These mice express GFP in all tissues examined. Expression levels vary between certain hematapoietic cell types. GFP expression is uniform within a cell type lineage and remains constant throughout development. T cells have a 2-fold higher GFP expression than CD19+B220+ B cells or peripheral blood cells. Leukocytes and red blood cells from homozygous transgenic mice fluoresce at approximately twice the level of cells from hemizygous mice.