FVB(B6)-Tg(Pdx1-cre)6Tuv/JwkimJ

Stock number: 034623
Product name: FVB.Pdx1-Cre
Strain synonyms: Pdx1-Cre on FVB

Research areas: Cancer Research, Research Tools

Description

These Pdx-Cre transgenic mice express Cre recombinase under the control of the mouse Pdx1 promoter on a congenic FVB/NJ background and may be useful for generating pancreas-specific conditional mutations.

Of note, The Jackson Laboratory Repository also offers Pdx1-cre mice on a C57BL/6 genetic background (Stock No. 014647).

Detailed description

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. The phenotype summarized below is for the parental line: Pdx1-Cre mice on a C57BL/6 genetic background (Stock No. 014647). It should be noted that the phenotype of this FVB/NJ-congenic Pdx1-Cre line (Stock No. 034623) could vary from that of the parental line from which it was derived.

Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of the mouse Pdx1 (pancreatic and duodenal homeobox 1) promoter. Mosaic Cre recombinase activity is detected in the pancreatic epithelium, antral stomach and duodenum in neonates and in pancreatic beta islet cells in adults. No Cre recombinase activity is detected ectopically to the Pdx1 expression domain. When crossed with a strain containing loxP site-flanked sequences, Cre-mediated recombination results in deletion of the floxed sequences in the cre-expressing tissues of the offspring. It is not known if homozygotes are viable.

Development

A transgenic construct containing sequence encoding Cre recombinase under the control of a 4.5 kb promoter fragment of mouse Pdx1 and a human ACTB 3' UTR followed by a poly A signal was introduced into FVB/N donor eggs. In founder line 6 the trangene integrated on chromosome 8, 48 kb upstream of Zfp423. The integration site contains a 1.5 kb genomic duplication. The donating investigator stated that these mice were backcrossed for 15 generations onto the C57BL/6 background before being donated to The Jackson Laboratory in 2011 as Stock No. 014647.

In 2013, Dr. Jung-Whan Kim purchased some Stock No. 014647 animals (~N17 onto C57BL/6) and then backcrossed them ten generations to FVB/NJ inbred mice (from Stock No. 001800). The resulting FVB/NJ-congenic strain is called FVB.Pdx1-Cre. Of note, the donating investigator reports that, at least once during backcrossing, a Pdx1-Cre female was bred to an FVB/NJ inbred male (thus the Y chromosome of the congenic strain is of FVB/NJ origin). In 2020, FVB.Pdx1-Cre males (white coat color) were sent to The Jackson Laboratory Repository as Stock No. 034623. Upon arrival, males were used to cryopreserve sperm. To establish our live colony, an aliquot of frozen sperm was used to fertilize FVB/NJ oocytes (Stock No. 001800).

Allele

Symbol: Tg(Pdx1-cre)6Tuv
Name: transgene insertion 6, David A Tuveson
MGI accession ID: MGI:3032531
Generation method: Transgenic
Attributes: Recombinase-expressing
Synonyms: PDX-1-Cre; Pdx1-cre; Pdx1-Cre^Tg; Pdx1-Cre^Tuv; Tg(Ipf1-cre)1Tuv; Tg(Ipf1-cre)6Tuv; Pdx1:Cre
Marker symbol: Tg(Pdx1-cre)6Tuv
Marker name: transgene insertion 6, David A Tuveson
Marker synonyms: Pdx-1-cre; Pdx1-cre; Tg(Ipf1-cre)1Tuv; Tg(Ipf1-cre)6Tuv
Chromosome: 8
Strain of origin: FVB/N
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: Cre expression is directed to islet cells of the pancreas.
Molecular note: The transgenic construct is composed of a Cre recombinase coding sequence under the control of the Pdx1 promoter. Cre expression is thus directed to islet cells of the pancreas. Line 6 was inserted into chromosome 8 about 48 kb upstream of Zfp423 (chr8:90,531,639; NCBI37/mm9) with elements of human ACTB 3'UTR and polyA signal, and a 1.5 kb genomic duplication at the integration site.