B6N.129-Ifi47^tm1Gat/J

Stock number: 034557
Product name: IRG-47 KO

Research areas: Immunology, Inflammation and Autoimmunity Research

Description

Interferon gamma inducible protein 47 (Ifi47) is a member of the IGTP family of genes which encode 47-48kD GTP-binding proteins upregulated by the activation of interferon gamma. These Ifi47 (also known as IRG-47 or Irgd) knock-out mice exhibit normal immune cell counts, but show increased susceptibility to infection by Toxoplasmsma gondii and may be useful for the study of immunology and infectious disease.

Detailed description

Interferon gamma inducible protein 47 (Ifi47) is a member of the IGTP family of genes which encode 47-48kD GTP-binding proteins transcriptionally upregulated by interferon gamma or LPS. The donating investigator indicates that mice homozygous for the deletion of Ifi47 have no overt abnormal phenotypes, are not significantly different in bodyweight from their wildtype counterparts, and reproduce in the expected Mendelian ratios. These animals have normal expression of myeloid and lymphoid cell populations, but show a moderately increased susceptibility to infection by the protozoan Toxoplasmsma gondii (67% mortality), which appears to manifest primarily during the chronic phase (10-47 days after infection) (Collazo et al. 2001). In contrast, these mice show no difference from wildtype in their susceptibility to Listeria monocytogenes or MCMV.

Development

The Ifi47^tm1Gat allele was created in the laboratory of Dr. Gregory A. Taylor at Duke University. A targeting vector was constructed to replace the entire coding region of the interferon gamma inducible protein 47 (Ifi47) gene with a PGK-neoBpA cassette. The construct was electroporated into 129/Sv-derived CJ7 embryonic stem (ES) cells and then microinjected into recipient blastocysts. The chimeric animals were crossed to C57BL/6NCrl mice for germline transmission and the line was subsequently backcrossed 8 generations to C57BL/6NCrl prior to being donated to The Jackson Laboratory in 2020. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6NJ oocytes (Stock No. 005304).

Allele

Symbol: Ifi47^tm1Gat
Name: targeted mutation 1, Gregory A Taylor
MGI accession ID: MGI:3027113
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Ifi47
Marker name: interferon gamma inducible protein 47
Marker synonyms: 47kDa; Igrd; Iigp4; IRG-47
Chromosome: 11
Strain of origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺
Molecular note: The entire coding region was replaced with a neomycin selection cassette inserted by homologous recombination. Western blot analysis of splenic and thymic lysates indicated an absence of protein in homozygous mutant mice.