Tlr9 cKO mutant mice possess loxP sites flanking exon 1 of the Tlr9 (toll like receptor 9) gene and may be useful in generating conditional mutations to study pathogen recognition and activation of innate immunity.
Louis De Muynck, Janssen R&D
TLR9 (toll-like receptor 9) is a transmembrane receptor, mainly expressed in the endocytic compartment, which plays a fundamental role in recognizing DNA that contains unmethylated CpG dinucleotides, and in the subsequent activation of innate immunity. This gene has been implicated in the pathogenesis of several autoimmune diseases.
These conditional Tlr9 cKO mice possess loxP sites flanking exon 1 of the Tlr9 gene. Mice that are homozygous for the mutation are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have the exon 1 deleted in cre-expressing tissues. These mice may be useful for studying pathogen recognition and activation of innate immunity.
Of note, the TLR9 KO is available as (Stock No. 034449)
CRISPR/Cas9 endonuclease-mediated genome editing was used to insert loxP sites flanking exon 1. A double guide RNA and Cas9 endonuclease mRNA were introduced into C57BL/6J-derived fertilized eggs by pronuclear injection along with a donor plasmid containing the Tlr9 exon 1 flanked in the 5' and 3' intronic sequences with a loxP sequence (ATAACTTCGTATAATGTATGCTATACGAAGTTAT). Embryos were transferred to pseudopregnant females, and correctly targeted pups were bred to C57BL/6J mice for germline transmission. Sequencing of N1 mice revealed the correct insertion of 5' and 3' loxP sites in cis. Upon arrival, mice were bred to C57BL/6J (Stock No. 000664) for at least 1 generation to establish the colony.
|Allele Name||endonuclease-mediated mutation 1, Louis De Muynck|
|Allele Type||Endonuclease-mediated (Conditional ready (e.g. floxed))|
|Gene Symbol and Name||Tlr9, toll-like receptor 9|
|Strain of Origin||C57BL/6J|
|Molecular Note||CRISPR/Cas9 endonuclease-mediated genome editing was used to insert loxP sites flanking exon 1.|
When maintaining a live colony, these mice are bred by homozygote sibling matings.
When using the Tlr9 cKO
mouse strain in a publication, please cite the originating article(s) and include JAX stock #034448 in your Materials and Methods section.