B6.129-Itgb1^tm1Efu/RthsnJ

Stock number: 034193
Product name: β1 integrin floxed
Strain synonyms: beta1^e3 , beta1^flox , CD29^f , integrin beta1^flox , Itgb1^f , Itgb1^flox
Research areas: Developmental Biology Research, Research Tools

Description

These floxed mutant mice possess loxP sites flanking exon 3 of the Itgb1 gene. This strain may be useful for generating conditional mutations in applications related to studies of epithelium and endothelium function and development.

Of note, a strain carrying the same allele on a B6;129 genetic background is available as Stock No. 004605.

Detailed description

These mice possess loxP sites on either side of exon 3 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Exposure to Cre recombinase results in a knock-out allele.

When bred to a strain expressing Cre recombinase in the epithelial cells of the intestine (see Stock No. 004586 for example), this mutant mouse strain may be useful in studies of intestinal hyperplasia.

When bred to a strain expressing Cre recombinase in the podocytes of the kidney glomeruli (see Stock No. 008205 for example), this mutant mouse strain may be useful in studies of glomerular structural integrity.

Of note, a strain carrying the same allele on a B6;129 genetic background is available as Stock No. 004605.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype of these B6J-congenic mice could vary from that originally described on a B6;129 genetic background. We may modify the strain description if necessary as published results become available.

Development

A loxP site flanked targeting vector containing neomycin resistance and phosphoglycerate kinase genes was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 3 of the targeted gene, and another loxP site was inserted upstream of exon 3. The construct was electroporated into 129X1/SvJ derived RW-4 embryonic stem (ES) cells which were transiently transfected with a vector containing the Cre-recombinase gene under the control of the cytomegalovirus promoter to remove the selection cassette. Correctly targeted ES cells were injected into C57BL/6J blastocysts. Chimeric mice were bred to C57BL/6J mice for one generation by the donating investigator before being sent to The Jackson Laboratory as Stock No. 004605. Some of these mice were obtained by Dr. David Rothstein (University of Pittsburgh) and were subsequently backcrossed to C57BL/6J mice (Stock No. 000664) for 10 generations to create this congenic strain.

Allele

Symbol: Itgb1^tm1Efu
Name: targeted mutation 1, Elaine Fuchs
MGI accession ID: MGI:1926499
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Synonyms: beta1^e3; beta1^flox; CD29^f; integrin beta1^flox; Itgb1^f; Itgb1^flox
Marker symbol: Itgb1
Marker name: integrin beta 1 (fibronectin receptor beta)
Marker synonyms: 4633401G24Rik; beta1 integrin; CD29; FNRB; Gm9863; GPIIA; MDF2; MSK12; VLAB; VLA-BETA
Chromosome: 8
Strain of origin: 129X1/SvJ
Molecular note: The targeting construct contains a loxP site upstream of exon 3 and a loxP-flanked neomycin selection cassette downstream of exon 3. Cre-mediated recombination removed the downstream floxed neo cassette leaving exon 3 floxed.