B6.129(Cg)-Lcp1^tm1.1Shcm/MgadJ

Stock number: 033973
Product name: LPL^fl
Research areas: Cell Biology Research, Developmental Biology Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

LPL^fl mice possess loxP sites flanking exon 2 of the lymphocyte cytosolic protein 1 (Lcp1) gene. These floxed mice are useful when studying motility, adhesion, and trafficking of hematopoietic cell lineages.

Detailed description

LPL^fl mice possess loxP sites flanking exon 2 of the lymphocyte cytosolic protein 1 (Lcp1) gene. LPL, a member of the alpha-actinin family, is expressed in hematopoietic cells. The roles of LPL in immune cell development and function vary with cell lineage. In neutrophils, LPL is dispensable for migration, adhesion, and spreading, but required for adhesion-mediated signaling to the oxidative burst. LPL is essential for normal lymphocyte motility and trafficking. In macrophages, LPL colocalizes with actin-supported structures and is concentrated in podosomes, which are integrin-anchored, actin-based complexes that support motility and adhesion. Mice that are homozygous for this floxed allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 2 deleted in the Cre-expressing tissues.

Development

The LPL^fl targeting vector was designed to insert a loxP site upstream of exon 2 and a frt-flanked neomycin selection cassette (in reverse orientation to the gene) followed by a second loxP site downstream of exon 2 of the lymphocyte cytosolic protein 1 (Lcp1) gene. The construct was electroporated into 129Sv/J embryonic stem (ES) cells and correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric mice were bred with FLP germline deleter mice on a C57BL/6 background to remove the neo cassette, and then the progeny were subsequently crossed to remove the FLP expressing transgene. The resulting LPL^fl colony was backcrossed to C57BL/6J mice for at least 6 generations. Upon arrival at The Jackson Laboratory, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

Allele

Symbol: Lcp1^tm1.1Shcm
Name: targeted mutation 1.1, Sharon Celeste Morley
MGI accession ID: MGI:6359802
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Synonyms: LPL^fl
Marker symbol: Lcp1
Marker name: lymphocyte cytosolic protein 1
Marker synonyms: CP64; D14Ertd310e; HEL-S-37; LC64P; L-fimbrin; L-plastin; PLS2
Chromosome: 14
Strain of origin: 129/Sv
Molecular note: A loxP site was inserted into intron 1 and an FRT site flanked neomycin resistance gene cassette plus a second loxP site were inserted into intron 2. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exon 2 floxed.
General note: The version of this allele that contains the neomycin selection cassette, Lcp1^tm1Shcm, has been analyzed.