B10Sn.LP-Zfp106^H3a-b B2m^a/DcrMmjax

Stock number: 033911
Product name: R19
Strain synonyms: R19
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

R19 is minor histocompatibility congenic strain that carries a portion of the H3 locus, Zfp106^H3a-b, derived from the LP strain on a C57BL/10Sn background. The strain may be useful for studying the role of minor histocompatibility genes in transplant rejection.

Detailed description

Zfp106 (formerly H3a) and H3b are minor histocompatibility genes that comprise the H3 locus. R19 is minor histocompatibility congenic strain that carries the closely linked Zfp106^H3a-b B2m^aalleles derived from the LP strain on a C57BL/10Sn background. The companion R03 strain (MMRRC 50798) carries the LP-derived Zfp106^H3a-b B2m^a and H3b^b alleles on a C57BL/10Sn background. Mice homozygous for the LP-derived alleles are viable and fertile.

Corneal allografts are one of the most common and successful tissue transplants in part because the cornea and anterior segment of the eye are immune privileged sites and do not require major histocompatibility complex (MHC) matching. However, those grafts that are rejected express minor histocompatibility (H) alloantigens. Corneal and skin allografts between R03 and R19 result in rejection of R03 donor corneas (expressing the H3b^b allele) by R19 recipients but not the reverse. R19 mice and R03 mice (MMRRC 50798) provide a tool to dissect the role of the minor H3 alloantigens in corneal and skin allografts.

Development

R19 is a minor histocompatibility congenic derived from B10.LP-H3^b/Sn, which contains a region on chromosome 2 derived from LP/DiSn that includes the closely linked Zfp106^H3a-b (formerly H3a) and B2m^a alleles. To create this strain B10.LP-H3^b/Sn mice were backcrossed to C57BL/10SnJ for an additional six generations narrowing the LP congenic region. Bracketing markers outside the LP congenic region are D2Mit395 to D2Mit304.

Allele

Symbol: B2m^a
Name: a variant
MGI accession ID: MGI:6357680
Generation method: Spontaneous
Attributes: Not Applicable
Marker symbol: B2m
Marker name: beta-2 microglobulin
Marker synonyms: AMYLD6; beta 2 microglobulin; beta2-m; IMD43; Ly-m11; MHC1D4
Chromosome: 2
Strain of origin: multiple strains
Molecular note: The a variant is slightly slower running on SDS PAGE and was found to have an aspartic acid at amino acid position 85. This allele is found in A, BALB/c, LP and most other strains.

Allele

Symbol: Zfp106^H3a-b
Name: histocompatibility 3a, b variant
MGI accession ID: MGI:6258284
Generation method: Spontaneous
Synonyms: H3a^b
Marker symbol: Zfp106
Marker name: zinc finger protein 106
Marker synonyms: Cd-1; H3a; SH3BP3; sirm; ZFP106; ZNF474
Chromosome: 2
Strain of origin: LP/J