Ccr10- knock-in/knock-out mice have an EGFP-neo cassette inserted into a mutated ATG site, abolishing endogenous gene expression. These mice may be useful for studying inflammation and lymphocyte homing.
Bao Lu, Children's Hospital Harvard Med School
Ccr10- knock-in/knock-out mice have an EGFP-neo cassette inserted into a mutated ATG site, abolishing endogenous gene expression. CCR10 encodes a chemokine receptor present on epidermal CD4 T cells that interacts with the ligand CCL27, which is highly expressed in the epidermis. This interaction is involved in T cell-mediated skin inflammation and lymphocyte homing.
These mice have a reduction of CD8+ T cells in the skin. EGFP can be detected by flow, and co-expresses with IgA antibody secreting cells. EGFP expression on other leukocyte subsets, such as neutrophils from the blood and spleen, which have not been shown to express CCR10, shows that this EGFP may be “leaky”.
The Ccr10- targeting vector was designed to mutate the ATG site of the chemokine (C-C motif) receptor 10 gene (Ccr10) to an EcoRV site, and an enhanced green fluorescent protein sequence (EGFP) fused to a neomycin resistance (neo) cassette, in reverse orientation to the gene, were inserted into the EcoRV site. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric mice were bred to BALB/cJ mice. These mice were backcrossed for 14 generations to BALB/cJ mice. Upon arrival at The Jackson Laboratory Repository, sperm was cryopreserved. An aliquot of frozen sperm was used to fertilize BALB/cJ oocytes (Stock No. 000651) to establish the colony.
|Allele Name||targeted mutation 1, Craig Gerard|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Ccr10tm1Cge; targeted mutation 1, Craig Gerard|
|Gene Symbol and Name||Ccr10, chemokine (C-C motif) receptor 10|
|Gene Synonym(s)||chemokine (C-C) receptor 9; GPR2; G protein-coupled receptor 2; Gpr2; Cmkbr9; Cmkbr9; CCR10; Gpr2|
|Strain of Origin||129S4/SvJae|
|Molecular Note||The first ATG codon of the gene was mutated to an EcoRV site, with an enhanced GFP gene and a neomycin resistance cassette being inserted into the EcoRV site. Gene inactivation was confirmed by a lack of product from RT-PCR of Peyer's patch RNA. GFP expression was observed in B cells expressing IgA, and in other leukocyte subsets including neutrophils.|
When maintaining a live colony, homozygous mice may be bred together.
When using the Ccr10- mouse strain in a publication, please cite the originating article(s) and include JAX stock #033905 in your Materials and Methods section.
|Heterozygous for Ccr10<tm1Cge>|
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