C.129S4-Ccr10^tm1Cge/BaoluJ

Stock number: 033905
Product name: Ccr10-
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

Ccr10^- knock-in/knock-out mice have an EGFP-neo cassette inserted into a mutated ATG site, abolishing endogenous gene expression. These mice may be useful for studying inflammation and lymphocyte homing.

Detailed description

Ccr10^- knock-in/knock-out mice have an EGFP-neo cassette inserted into a mutated ATG site, abolishing endogenous gene expression. CCR10 encodes a chemokine receptor present on epidermal CD4 T cells that interacts with the ligand CCL27, which is highly expressed in the epidermis. This interaction is involved in T cell-mediated skin inflammation and lymphocyte homing.

These mice have a reduction of CD8+ T cells in the skin. EGFP can be detected by flow, and co-expresses with IgA antibody secreting cells. EGFP expression on other leukocyte subsets, such as neutrophils from the blood and spleen, which have not been shown to express CCR10, shows that this EGFP may be “leaky”.

Development

The Ccr10^- targeting vector was designed to mutate the ATG site of the chemokine (C-C motif) receptor 10 gene (Ccr10) to an EcoRV site, and an enhanced green fluorescent protein sequence (EGFP) fused to a neomycin resistance (neo) cassette, in reverse orientation to the gene, were inserted into the EcoRV site. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric mice were bred to BALB/cJ mice. These mice were backcrossed for 14 generations to BALB/cJ mice. Upon arrival at The Jackson Laboratory Repository, sperm was cryopreserved. An aliquot of frozen sperm was used to fertilize BALB/cJ oocytes (Stock No. 000651) to establish the colony.

Allele

Symbol: Ccr10^tm1Cge
Name: targeted mutation 1, Craig Gerard
MGI accession ID: MGI:3036706
Generation method: Targeted
Attributes: Null/Knockout
Synonyms: CCR10 -
Marker symbol: Ccr10
Marker name: chemokine (C-C motif) receptor 10
Marker synonyms: CCR10; Cmkbr9; GPR2
Chromosome: 11
Strain of origin: 129S4/SvJae
Site of expression: GFP expression is observed in B cells expressing IgA, and in other leukocyte subsets including neutrophils.
Molecular note: The first ATG codon of the gene was mutated to an EcoRV site, with an enhanced GFP gene and a neomycin resistance cassette being inserted into the EcoRV site. Gene inactivation was confirmed by a lack of product from RT-PCR of Peyer's patch RNA. GFP expression was observed in B cells expressing IgA, and in other leukocyte subsets including neutrophils.