Mice homozygous for the targeted mutation are viable and fertile. No transcripts from the targeted gene are detected in bone marrow. Homozygous mice have normal T and B cell development but elevated IgG1 and decreased IgG2a, IgG3, and IgA in serum. In standard models of airway hyperresponsiveness (intraperitoneal (i.p.) sensitization followed by aerosol challenge), mutant mice are protected from allergic airway disease. Following epicutaneous, but not i.p., sensitization, homozygotes have significantly greater serum IgG1, dermal eosinophilia, and splenocyte Th2 cytokine secretion. Antigen presenting cells from null mice induce stronger Th2 responses. This mutant may be suitable for use in studies related to asthma, allergic skin disease, T helper cell polarization/B cell isotype switching, and other Th2- and innate-immunity studies. Of note, this allele is also available on a congenic BALB/cJ background as Stock No. <a href="https://www.jax.org/strain/005712">005712</a>. In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype of these C57BL/6J congenic mice could vary from that originally described on a BALB/c congenic background. We may modify the strain description if necessary as published results become available.

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Homozygous C3ar1tm1Cge knock-out mice have elevated IgG1 and decreased IgG2a, IgG3, and IgA in serum. This mutant may be suitable for use in studies related to asthma, allergic skin disease, and innate-immunity studies.

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