R26DM.lsl-MYC mice have a floxed-STOP cassette preventing expression of a downstream human MYC (cDNA) sequence. When R26DM.lsl-MYC mice are crossed with a Cre recombinase strain, the floxed-STOP cassette is deleted and the Gt(ROSA)26Sor promoter drives expression of human MYC in the cre-expressing cells. These mice are useful in studies related to tumorigenesis.
Daniel J. Murphy, University of Glasgow
R26DM.lsl-MYC mice express Cre recombinase-inducible human MYC from the endogenous Gt(ROSA)26Sor locus. When these R26DM.lsl-MYC mice are crossed with a Cre recombinase strain, the floxed-STOP cassette is deleted and the Gt(ROSA)26Sor promoter drives expression of human MYC (cDNA) in the cre-expressing cells. The donating investigator reports expression levels of human MYC at approximately physiological levels that are insufficient to induce apoptosis in most adult tissues. Human MYC expression levels were assayed in lung, pancreas, liver by PCR, in situ hybridization, and/or immunohistochemistry, and in MEFs by qPCR and immunoblot. Homozygotes are viable and fertile. During backcrossing, the Y chromosome may not have been fixed to the FVB/N genetic background.
When crossed to B6.129S4-Krastm4Tyj/J mice (Stock No. 008179) the resulting double mutant mice are the Cre-inducible KM model of lung adenocarcinoma.
A targeting vector containing a floxed STOP cassette (with neo cassette and triple polyadenylation sequence), followed by human MYC cDNA was inserted into the Gt(ROSA)26Sor locus via electroporation into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were tested for germline transmission, and then backcrossed to FVB/N for more than 10 generations. During backcrossing, the Y chromosome may not have been fixed to the FVB/N genetic background. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize FVB/NJ oocytes (Stock No. 001800).
Currently there are no related genes or alleles for this strain.
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Rosa26^DM-lsl-MYC , Rosa26-lsl-MYC , R26DM.lsl-MYC mouse strain in a publication, please cite the originating article(s) and include JAX stock #033805 in your Materials and Methods section.
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