C57BL/6-Nxph4^{tm1a(KOMP)Wtsi}/HzoJ

Stock number: 033791
Product name: Nxph4^βgeo
Research areas: Neurobiology Research, Research Tools

Description

Nxph4^βgeo knock-in/knock-out mice express lacZ from the mouse Nxph4 (neurexophilin 4) promoter in tissues that normally express the gene. The strain can be used to study Nxph4 expression and its loss-of-function effect.

Detailed description

The Nxph4^βgeo knock-in/knock-out mice express lacZ from the mouse Nxph4 (neurexophilin 4) promoter in tissues that normally express the gene, including a small population of brain cells in cortical layer 6b and cerebellar inhibitory neurons, as well as the spinal cord. Heterozygotes and homozygotes are viable and fertile, and live a normal lifespan. Both female and male homozygotes show reduced body weight and anxiety, motor incoordination that does not affect locomotor function, and, in male mice, reduced pre-pulse inhibition. The strain can be used to study Nxph4 expression and its loss-of-function effect.

Development

Embryonic stem (ES) cells carrying the Nxph4^{tm1a(KOMP)Wtsi} knock-out first allele with a promoter-driven selection cassette were acquired from KOMP. This allele, incorporating an FRT-lacZ-loxP-neomycin-FRT-loxP cassette in intron 1 and a loxP site in intron 2 of the mouse Nxph4 gene, was created in JM8A1.N3 C57BL/6N-A^tm1Brd-derived ES cells. Resultant chimeric mice were crossed with albino C57BL/6J mice to detect germline transmission. The donating laboratory subsequently bred the line to C57BL/6J mice for an unknown number of generations. The albino and agouti (A^tm1Brd) alleles have likely been bred out of the line.

Allele

Symbol: Nxph4^{tm1a(KOMP)Wtsi}
Name: targeted mutation 1a, Wellcome Trust Sanger Institute
MGI accession ID: MGI:4363267
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); Reporter; Null/Knockout
Synonyms: Nxph4^betageo
Marker symbol: Nxph4
Marker name: neurexophilin 4
Marker synonyms: 1110036M10Rik; Nph4
Chromosome: 10
Strain of origin: C57BL/6N-A^tm1Brd
Molecular note: The L1L2_Bact_P cassette was inserted at position 127361075 of Chromosome 10 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 127363252. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml