B6J.129S4-Bdnf^tm3Jae/RujfJ

Stock number: 033689
Product name: Bdnf^2lox
Research areas: Apoptosis Research, Neurobiology Research, Research Tools, Sensorineural Research

Description

The coding region of the mouse Bdnf (brain derived neurotrophic factor) is flanked by loxP sites in these Bdnf^2lox mice. Cre recombinase-mediated excision of the floxed region results in a knock-out allele.

This line has been backcrossed to C57BL/6J for more than 10 generations by the donating laboratory

Detailed description

The coding region of the mouse Bdnf (brain derived neurotrophic factor) is flanked by loxP sites in these Bdnf^2lox mice. Cre recombinase-mediated excision of the floxed region results in a knock-out allele.

When bred to a strain expressing Cre recombinase under the control of a tetracycline-responsive promoter element (see Stock No. 006224, 006234, 006244) and a strain expressing a tetracycline-controlled activator protein in brain tissues (see Stock No. 003763), this mutant mouse strain may be useful in studies of hippocampal-dependent learning and long-term potentiation. When bred to a strain expressing Cre recombinase in neuronal cells (see Stock No. 003966 for example), this mutant mouse strain may be useful in studies of epilepsy.

This line has been backcrossed to C57BL/6J for more than 10 generations by the donating laboratory.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

A single loxP site was introduced upstream of the Bdnf coding region, and a loxP-flanked hygromycin resistance (hygro) cassette was placed downstream of the coding region via homologous recombination in 129S4/SvJae derived J1 embryonic stem (ES) cells.  Transient expression of a Cre recombinase vector in the ES cells removed the hygro cassette, leaving the coding region flanked by loxP sites. This line has been backcrossed to C57BL/6J for more than 10 generations by the donating laboratory.

Allele

Symbol: Bdnf^tm3Jae
Name: targeted mutation 3, Rudolf Jaenisch
MGI accession ID: MGI:2176750
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Synonyms: Bdn^flox; Bdnf²; Bdnf^2L; Bdnf^2lox; Bdnf^tm2Jae
Marker symbol: Bdnf
Marker name: brain derived neurotrophic factor
Marker synonyms: ANON2; BULN2
Chromosome: 2
Strain of origin: 129S4/SvJae
Molecular note: A single loxP site was inserted 5' to the only coding exon, and a loxP-flanked hygromycin resistance cassette was inserted 3' to the exon. The hygromycin selection cassette was removed in ES cells by transient Cre expression leaving a single loxP site prior to the production of chimeric mice.