GnRH-EGFP mice express an enhanced green fluorescent protein (EGFP) in GnRH-expressing neurons found in the medial preoptic area and hypothalamus. This mutant mouse strain may be useful in visualizing the structure and function of gonadotropin-releasing hormone neurons.
Suzanne Moenter, University of Michigan
Neurons that synthesize and secrete Gnrh1 (gonadotropin releasing hormone 1) integrate and control peripheral and central aspects of reproduction, including the onset of puberty. The hormone is produced by a small and diffuse population of neurons scattered in the rostral hypothalamus and medial preoptic area (MPOA) and in the basal forebrain, including the septum and diagonal band of Broca. The GnRH-EGFP transgene uses the mouse Gnrh1 (gonadotropin releasing hormone 1) promoter to express the enhanced green fluorescent protein (EGFP) in GnRH-expressing neurons. EGFP expression mimics the endogenous promoter expression pattern and allows for identification of 84-94% of GnRH neurons in hemizygous mice. In some mice, ectopic expression can be observed in the lateral septum, which expresses GnRH during development. Mice homozygous for the transgene are viable and fertile. This mutant mouse strain may be useful in visualizing the structure and function of gonadotropin-releasing hormone neurons.
The transgenic construct contains a portion of the mouse Gnrh1 (gonadotropin releasing hormone 1) promoter (-3446 to +23) and the B intron of the rabbit β-globin gene directing expression of enhanced green fluorescent protein (EGFP) to GNRH1-expressing neurons. The linearized construct was microinjected into fertilized (CBA x C57BL/6)F1 oocytes. Founder line 51 was subsequently established. Founder animals were bred to C57BL/6J mice and then backcrossed to the same for at least ten generations by the donating lab (see SNP note attached). Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
A 48 SNP (single nucleotide polymorphism) panel analysis, with 43 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. Six of the 43 markers throughout the genome were segregating with CBA, suggesting an incomplete backcross.
|Allele Name||transgene insertion 51, Suzanne Moenter|
|Allele Type||Transgenic (Reporter)|
|Allele Synonym(s)||GnRH-GFP transgenic; Tg(Gnrh1-EGFP)1Sumo|
|Gene Symbol and Name||Tg(Gnrh1-EGFP)51Sumo, transgene insertion 51, Suzanne Moenter|
|Site of Expression||GnRH-expressing neurons found in the medial preoptic area and hypothalamus|
|Strain of Origin||(CBA x C57BL/6)F1|
|Molecular Note||The mouse promoter (-3446 to +23) drives expression of an EGFP reporter gene preceded by a the B intron of rabbit beta-globin (600 bp) and followed by a human growth hormone polyadenylation signal. Two lines were generated. Expression is detected in the medial preoptic area and hypothalamus at 21 and 60 days of age.|
When maintaining a live colony, these mice are bred by homozygote sibling matings.
When using the GnRH-GFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #033639 in your Materials and Methods section.