B6(Cg)-Il7^{tm1d(EUCOMM)Wtsi}/BsedJ

Stock number: 033588
Product name: Il7^KO
Research areas: Developmental Biology Research, Immunology, Inflammation and Autoimmunity Research

Description

Il7^KO mice have exons 3-4 of the interleukin 7 (Il7) gene removed, making these mice useful for studying the development and maintenance of the lymphoid system.

Detailed description

Il7^KO mice have exons 3-4 of the interleukin 7 (Il7) gene removed. IL7 is a cytokine important for B and T cell development and plays an essential role in lymphoid cell survival. Mice that are homozygous for this allele are viable and fertile. Mice are lymphopenic, with reduced numbers of peripheral T and B cells in the lymph nodes and spleen. Thymi are also small and exhibit a developmental block at the DN2 stage.

Of note, the floxed version of this allele is available in Stock No. 033410.

Development

The L1L2_Bact_P cassette was inserted at a position on Chromosome 3 upstream of exon 3 of the interleukin 7 (Il7) gene. The cassette is composed of an FRT site followed by a lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance cassette under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site was inserted downstream of exon 4. The construct, clone EPD0609_3_H09, was introduced into C57BL/6N-A^tm1Brd-derived JM8A3.N1 embryonic stem (ES) cells, and correctly targeted ES cells were injected into blastocysts. The resulting chimeric mice were bred to ACTFLPe mice (Stock No. 003800) to remove the FRT-flanked lacZ sequence and neomycin cassette. Resulting Il7^flox/flox offspring were bred to C57BL/6J mice (Stock No. 000664) for at least 5 generations. Upon arrival at The Jackson Laboratory Repository, sperm was cryopreserved. An aliquot of frozen sperm was used to fertilize C57BL/6J oocytes to establish our live colony of Il7^flox/flox mice as Stock No. 033410.

An aliquot of frozen sperm was also used to fertilize oocytes from Sox2Cre mice (Stock No. 008454) to remove exons 3-4, resulting in this colony of Il7^KO mice.

Allele

Symbol: Il7^{tm1d(EUCOMM)Wtsi}
Name: targeted mutation 1d, Wellcome Trust Sanger Institute
MGI accession ID: MGI:6388790
Generation method: Targeted
Attributes: Null/Knockout
Synonyms: Il7^KO
Marker symbol: Il7
Marker name: interleukin 7
Marker synonyms: A630026I06Rik; hlb368; IL-7; IMD130
Chromosome: 3
Strain of origin: C57BL/6N-A^tm1Brd
Molecular note: The L1L2_Bact_P cassette was inserted at a position on Chromosome 3 upstream of exon 3. The cassette is composed of an FRT site followed by a lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance cassette under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site was inserted downstream of exon 4. Flp-mediated recombination removed the FRT-flanked lacZ/neo cassette. Cre-mediated recombination removed exons 3 and 4.