NCoR1L2 mice possess loxP sites flanking exon 11 of the nuclear receptor co-repressor 1 (Ncor1) gene. These floxed mice are useful when studying the role of NCoR1 as a negative transcriptional regulator.
Johan Auwerx, École polytechnique fédérale de Lausanne
NCoR1L2 mice possess loxP sites flanking exon 11 of the nuclear receptor co-repressor 1 (Ncor1) gene. NCoR1 is a transcriptional repressor involved in metabolism, cellular development, and cancer. Mice that are homozygous for this floxed allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 11 deleted in the cre-expressing tissues.
For example, when bred to Lck-Cre mice (Stock No. 003802), expressing cre recombinase in thymocytes, resulting knock-out offspring have reduced mature thymocyte numbers.
The NCoR1L2 targeting vector was designed to insert a loxP site upstream of exon 11, and loxP-flanked and frt-flanked neomycin selection cassette, downstream of exon 11 of the nuclear receptor co-repressor 1 (Ncor1) gene. The construct was electroporated into 129Sv-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts and resulting chimeric males were bred with ACTFLPe mice on a C57BL/6J background (Stock No. 005703) to remove the neo cassette. Progeny were crossed to remove the Flpe-expressing transgene and mice were subsequently bred to C57BL/6J mice for at least 15 generations. Upon arrival at The Jackson Laboratory, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
|Allele Name||targeted mutation 1.1, Mouse Clinical Institute|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Ncor1, nuclear receptor co-repressor 1|
|Strain of Origin||129|
|Molecular Note||A loxP site was inserted upstream of exon 11 and a loxP and FRT flanked neo cassette was inserted downstream of exon 11 via homologous recombination. Flp mediated recombination removed the neo cassette leaving exon 11 floxed.|
When maintaining a live colony, homozygous mice may be bred together.
When using the NCoR1L2 mouse strain in a publication, please cite the originating article(s) and include JAX stock #033452 in your Materials and Methods section.
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