NtsR2Cre mice have a Cre Recombinase sequence downstream of the stop codon of the neurotensin receptor 2 (Ntsr2) gene. These mice may be useful as a reporter when studying developmental versus adult expression patterns of NtsR2.
Gina Leinninger, Michigan State University
NtsR2Cre mice have a Cre Recombinase sequence downstream of the stop codon of the neurotensin receptor 2 (Ntsr2) gene. Neurotensin is a neuropeptide expressed throughout the central nervous system and has been linked to numerous physiologic and behavioral processes including feeding, body temperature, nociception, emotional state, and sleep. NtsR2 is a G protein-coupled receptor for Neurotensin that is expressed in glial cells in the ventral tegmental area (VTA) of the brain and elsewhere. Mice that are heterozygous for this allele are viable and fertile, the donating investigator did not attempt to make a homozygous colony.
When these mice are crossed to a strain containing a floxed sequence, Cre expressing cells can be identified during development. Specifically, expression is evident in glial cells during development.
The NtsR2Neo-Cre targeting vector was designed to insert a frt-flanked STOP cassette, including a neomycin resistance (neo) cassette, and an internal ribosome entry site (IRES) fused to a Cre Recombinase sequence downstream of the stop codon of the neurotensin receptor 2 (Ntsr2) gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. ES cells were injected into C57BL/6 blastocysts and resulting chimeric males were mated to C57BL/6J females. NtsR2Neo-Cre mice were subsequently bred to ROSA26::FLPe mice (Stock No. 009086) to remove the STOP cassette, and then backcrossed to C57BL/6J mice for at least 6 generations. Upon arrival at The Jackson Laboratory Repository, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
|Allele Name||targeted mutation 1.1, Gina M Leinninger|
|Allele Type||Targeted (Recombinase-expressing)|
|Allele Synonym(s)||NtsR2deltaNEO-Cre; NtsR2Dev|
|Gene Symbol and Name||Ntsr2, neurotensin receptor 2|
|Site of Expression||Expressed throughout the central nervous system.|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||The targeting vector was generated by inserting an IRES-Cre between the stop codon and the polyadenylation site of the sequence encoding the 3' end of the mouse NtsR2 gene, with an FRT-flanked NEO cassette placed upstream of the IRES-Cre. Correctly recombined ES clones via homologous recombination were expanded, and injected into mouse blastocystes to generate chimeric males. Mice were bred to a FlpO deleter line to remove neo cessette to make constitutive cre expression throughout life.|
When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony, or to C57BL/6J inbred mice (Stock No. 000664). The donating investigator did not attempt to make a homozygous colony.
When using the NtsR2NEO-Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #033394 in your Materials and Methods section.