PLP-EGFP mice express an enhanced green fluorescent protein (EGFP) in oligodendroctyes, Schwann cells and the enteric glia of the gut. Expression occurs in embryonic and postnatal cells. This mutant mouse strain may be useful in visualizing all stages of oligodendrocyte differentiation.
Wendy Macklin, University of Colorado
The PLP-EGFP transgene uses the mouse Plp1, proteolipid protein (myelin) 1 promoter to direct expression of enhanced green fluorescent protein (EGFP) to oligodendroctyes, Schwann cells and the enteric glia of the gut. EGFP expression occurs in embryonic tissue from at least E10.5 and postnatal tissue, and mimics the endogenous promoter expression pattern. PLP1 is the predominant component of myelin. Mice hemizygous for the transgene are viable and fertile. This mutant mouse strain allows visualization of all stages of the oligodendrocyte lineage.
The transgenic construct contains the mouse proteolipid protein (myelin) 1 (Plp1) promoter regulatory elements and the Plp1 3' untranslated region ligated to EGFP sequence and injected into C57BL/6 x CBA fertilized oocytes. Founder line 10 was subsequently established. Founder animals were bred to C57BL/6J mice and then backcrossed to the same for at least four generations by the donating lab (see SNP note below). Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
In 2020, a 48 SNP (single nucleotide polymorphism) panel analysis, with 43 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. Two of the 43 markers, on Chromosomes 16 and 18, were segregating with CBA, suggesting an incomplete backcross.
|Allele Name||transgene insertion 10, Wendy Macklin|
|Allele Type||Transgenic (Reporter)|
|Gene Symbol and Name||Tg(Plp1-EGFP)10Wmac, transgene insertion 10, Wendy Macklin|
|Site of Expression||oligodendroctyes, Schwann cells and the enteric glia of the gut|
|Strain of Origin||C57BL/6 x CBA|
|Molecular Note||A mouse Plp1 promoter was used to drive expression of EGFP. Three lines were produced, EGFP3, EGFP5, and EGFP10.|
Hemizygotes are viable and fertile. The donating investigator has not attempted to generate homozygous mice.
When using the PLP-EGFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #033357 in your Materials and Methods section.