GrprF mice possess loxP sites flanking exon 2 of the X-linked gastrin releasing peptide receptor (Grpr) gene. These floxed mice are useful when studying contagious itch behavior.
Zhou-Feng Chen, Washington University School of Medicine
GrprF mice possess loxP sites flanking exon 2 of the X-linked gastrin releasing peptide receptor (Grpr) gene. GRPR is a glycosylated, 7-transmembrane G-protein coupled receptor that activates numerous functions of the gastrointestinal and central nervous systems, including release of gastrointestinal hormones, smooth muscle cell contraction, and epithelial cell proliferation and is a potent mitogen for neoplastic tissues. GRPR has been shown to transmit contagious itch information in the suprachiasmatic nucleus (SCN) of the hypothalamus. Homozygous females and hemizygous males are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues.
When injected with a viral vector encoding Cre recombinase (cre) and enhanced green fluorescent protein (AAV2-Syn-Cre-IRES-eGFP) bilaterally into the SCN of the hypothalamus, contagious scratching behavior was abolished.
The GrprF targeting vector was designed to insert a loxP site upstream of exon 2, and an FRT-flanked neomycin selection cassette followed by a second loxP site downstream of exon 2 of the X-linked gastrin releasing peptide receptor (Grpr) gene. The construct was electroporated into 129S7/SvEvBrd-Hprt+-derived AB1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred with C57BL/6J mice for at least two generations. Upon arrival at The Jackson Laboratory, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
|Allele Name||targeted mutation 1, Zhou-Feng Chen|
|Allele Type||Targeted (Conditional ready (e.g. floxed))|
|Gene Symbol and Name||Grpr, gastrin releasing peptide receptor|
|Strain of Origin||129S7/SvEvBrd-Hprt+|
|Molecular Note||A loxP site was inserted approximately 0.5 kb upstream of exon 2 and an FRT-flanked neomycin resistance gene and a loxP site were inserted about 0.8 kb downstream of exon 2.|
When maintaining a live colony, homozygous females may be bred to hemizygous males.
When using the GrprF mouse strain in a publication, please cite the originating article(s) and include JAX stock #033148 in your Materials and Methods section.
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