B6.129S-Pdgfra^{tm1.1(cre/ERT2)Blh}/J

Stock number: 032770
Product name: Pdgfrα-CreER^T2
Research areas: Research Tools

Description

Pdgfrα-CreER^T2 mice have a cre/ERT2 fusion gene inserted into the start codon of the platelet derived growth factor receptor, alpha polypeptide (Pdgfra) gene. Insertion of this fusion gene abolishes Pdgfrα gene expression.

Detailed description

Pdgfrα-CreER^T2 mice have a cre/ERT2 fusion gene inserted into the start codon of the platelet derived growth factor receptor, alpha polypeptide (Pdgfra) gene. Insertion of this fusion gene abolishes Pdgfrα gene expression. Pdgfrα encodes a cell surface tyrosine kinase receptor expressed in a variety of cell types. It is involved in organ development, differentiation of bone marrow-derived mesenchymal cells, and normal development of the mucosa lining the gastrointestinal tract and development of the lung. It also plays a role in cell migration, chemotaxis in wound healing, and tumor progression. Mice that are heterozygous for this knock-in/knock-out allele are viable and fertile, while homozygotes are not viable.

When Pdgfrα-CreER^T2 mice are bred with mice containing loxP-flanked sequence(s), tamoxifen-inducible Cre-mediated recombination will result in deletion of the floxed sequence(s) in the Cre-expressing cells of the offspring. Cre recombination occurs in Pdgfra⁺ fibroblasts in many tissues in the developing embryo and adult mouse after tamoxifen administration.

 

Development

The Pdgfrα-CreER^T2 targeting vector was designed to insert a cre/ERT2 fusion gene (Cre recombinase fused to a human estrogen receptor ligand binding domain), followed by a polyadenylation signal and a frt-flanked neomycin resistance cassette, into the start codon of the platelet derived growth factor receptor, alpha polypeptide (Pdgfra) gene. The construct was electroporated into 129S6/SvEvTac-derived TL1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric mice were bred with 129S4-Gt(ROSA)26Sor^tm2(FLP*)Sor/J mice (Stock No. 007844) to remove the neo cassette. Resulting Pdgfrα-CreER^T2 mice were bred to C57BL/6 mice for at least 15 generations. Upon arrival at The Jackson Laboratory, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

A 48 SNP (single nucleotide polymorphism) panel analysis, with 43 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. One of the 43 markers on Chromosome 6 was still segregating with 129. Also, 1 of the 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6N;C57BL/6J genetic background.

Allele

Symbol: Pdgfra^{tm1.1(cre/ERT2)Blh}
Name: targeted mutation 1.1, Brigid L Hogan
MGI accession ID: MGI:6201731
Generation method: Targeted
Attributes: Recombinase-expressing; Inducible
Synonyms: Pdgfra-CreER^T2
Marker symbol: Pdgfra
Marker name: platelet derived growth factor receptor, alpha polypeptide
Marker synonyms: APDGFR; CD140a; PDGFACE; PDGFR2; PDGFR-2
Chromosome: 5
Strain of origin: 129S6/SvEvTac
Site of expression: Fibroblasts in many tissues in the developing embryo and adult.
Molecular note: The targeting vector contained an cre/ERT2 cassette inserted at the start codon of the Pdgfra locus. The cre cassette was followed by a FRT-flanked neo cassette, which was removed by flp-mediated excision after germline transmission.