STOCK Tg(ARR2/Pbsn-MYC)7Key/AbshnJ

Stock number: 032754
Product name: Hi-Myc
Research areas: Cancer Research, Internal/Organ Research, Research Tools

Description

Hi-Myc mice over-express a flag-tagged human MYC in the prostate. These mice serve as a good tool to study the progression of prostate cancer in a mouse model.

Detailed description

Hi-Myc mice over-express a flag-tagged human myelocytomatosis oncogene (MYC) cDNA under the control of a truncated rat probasin (Pbsn) promoter with 2 additional androgen-inducible elements. Expression is evident in the prostate. These mice serve as a good tool to study the progression of prostate cancer in a mouse model.

Development

The Hi-Myc transgene was designed with nucleotides -426 through +28 of the rat probasin (Pbsn) gene, containing its androgen-inducible, prostate-specific promoter and 2 additional androgen-inducible elements, directing expression of a flag-tagged human MYC cDNA followed by a poly-adenylation signal. This transgene was microinjected into fertilized FVB oocytes. Hi-Myc mice from founder line 7 were maintained on an FVB/N genetic background. Upon arrival at The Jackson Laboratory, this line was maintained with other alleles before this allele was separated out, creating mice sustained as this single allelic strain.

Allele

Symbol: Tg(ARR2/Pbsn-MYC)7Key
Name: transgene insertion 7, Katharine Ellwood-Yen
MGI accession ID: MGI:3785495
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Synonyms: ARR2/probasin-Myc; ARR2PB-Myc-PAI; Hi-Myc; Tg(Pbsn-MYC)4Key
Marker symbol: Tg(ARR2/Pbsn-MYC)7Key
Marker name: transgene insertion 7, Katharine Ellwood-Yen
Marker synonyms: ARR2PB-Myc-PAI; Hi-Myc; Tg(Pbsn-MYC)4Key
Chromosome: UN
Strain of origin: FVB
Site of expression: Prostate
Molecular note: Nucleotides -426 through +28 of the rat probasin gene, containing its androgen-inducible, prostate-specific promoter with 2 additional androgen-inducible elements, were joined to a human MYC cDNA flanked by a FLAG epitope coding sequence and the poly-adenylation signal from the insulin receptor gene. Immunoblot analysis with an antibody specific for human MYC demonstrates expression in prostate of mice as young as two weeks of age, which increases as the mice approach puberty at approximately 4 weeks. Four founder lines were established (4, 7, 11 and 13) since no distinction was made between the lines, line 7 was chosen as the representative line.