STOCK Nkx3-1^{tm4(cre/ERT2)Mms}/AbshnJ

Stock number: 032753
Product name: Nkx3-1^CreERT2
Research areas: Research Tools

Development

The Nkx3.1CreERT2 targeting vector was designed to insert a cre/ERT2 fusion gene (Cre recombinase fused to a human estrogen receptor ligand binding domain) followed by a polyadenylation sequence into the translation start site of the NK3 homeobox 1 (Nkx3-1) gene. The construct also included a self-excising ACN cassette for positive selection and a PGK-tk (thymidine kinase) cassette for negative selection. The ACN cassette, containing the neomycin resistance gene and Cre recombinase gene under the control of angiotensin-converting enzyme promoter, is flanked by loxP sites. Cre-mediated recombination during spermatogenesis removes the cassette leaving one loxP site. The construct was electroporated into 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred with C57BL/6 mice to establish a colony. This line of Nkx3.1^CreERT2 mice were maintained with other alleles before this allele was separated out, creating mice sustained as this single allelic strain.

Allele

Symbol: Nkx3-1^{tm4(cre/ERT2)Mms}
Name: targeted mutation 4, Michael M Shen
MGI accession ID: MGI:4365630
Generation method: Targeted
Attributes: Recombinase-expressing; Inducible; Null/Knockout
Synonyms: Nkx3-1^CreERT2
Marker symbol: Nkx3-1
Marker name: NK3 homeobox 1
Marker synonyms: bagpipe; BAPX2; Bax; NKX3; Nkx3.1; Nkx-3.1; NKX3A
Chromosome: 14
Strain of origin: 129S6/SvEvTac
Site of expression: Prostate.
Molecular note: A cre/ERT2-polyA sequence was inserted at the translation start site of the Nkx3-1 gene, thus generating a null allele. The targeting vector also contained a self-excising ACE-cre/PolII-neo selection cassette for positive selection and a PGK-tk (thymidine kinase) cassette for negative selection. The vector was transfected into TC-1 ES cells which were used to generate chimeras. Excision of the selection cassette occurred by passage through the male germline.