MFN2WT mice express flag-tagged wild-type human mitofusin 2 (MFN2) under direction of the murine Thy1 promoter. These mice may be useful when studying Charcot-Marie-Tooth Neuropathy Type 2 (CMT2).
Robert H Baloh, Cedars Sinai Medical Center
MFN2WT transgenic mice contain a murine thymus cell antigen 1, theta (Thy1; Thy1.2) promoter driving expression of a flag-tagged wild-type human mitofusin 2 (MFN2) gene in the nervous system. Mutations in the MFN2 GTPase domain have been associated with the onset of Charcot-Marie-Tooth disease type 2A (CMT2A) across numerous ethnic backgrounds with a consistently severe early-onset phenotype that, in addition to peripheral neuropathy, may include optic atrophy, spasticity, developmental delay, and brain white matter abnormalities. Homozygous mice are viable and fertile. These mice are indistinguishable from non-transgenic littermates, and may be a suitable control for the MFN2R94Q:MFN1 double transgenic strain (Stock No. 033391).
The 9102 bp MFN2WT transgenic construct was designed to contain a murine thymus cell antigen 1, theta (Thy1; Thy1.2) promoter driving expression of a flag-tagged wild-type human mitofusin 2 (MFN2) gene. The linearized construct was microinjected into fertilized C57BL/6J oocytes. Founder line 47 was bred to and maintained on a C57BL/6J genetic background. Upon arrival at The Jackson Laboratory, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
|Allele Name||transgene insertion 47, Robert Baloh|
|Allele Type||Transgenic (Inserted expressed sequence, Humanized sequence, Epitope tag)|
|Gene Symbol and Name||Tg(Thy1-MFN2)47Balo, transgene insertion 47, Robert Baloh|
|Strain of Origin||C57BL/6J|
|Molecular Note||The 9102 bp transgenic construct is designed to contain a murine thymus cell antigen 1, theta (Thy1; Thy1.2) promoter driving expression of a flag-tagged human mitofusin 2 (MFN2) gene.|
When maintaining a live colony, hemizygous mice may be bred together. The donating lab has not attempted to make a homozygous colony.
When using the MFN2WT mouse strain in a publication, please cite the originating article(s) and include JAX stock #032728 in your Materials and Methods section.
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