T2 mice have a Tet-responsive element (TRE or tetO) and mouse prion protein promoter sequences (PrP or Prnp) directing expression of the pathogenic P301L mutant variant of human microtubule-associated protein tau (MAPT) inserted into an intergenic site downstream of the Col1a1 locus. The tauP301L construct is the same as the construct used to create Tg4510 (Stock No. <a href="https://www.jax.org/strain/015815"">015815</a>); however, the T2 allele does not affect expression of any endogenous genes. Mice homozygous for the T2 allele are viable and fertile. When T2 mice are exposed to tetracycline-controlled transactivator protein (tTA) or reverse tetracycline-controlled transactivator protein (rtTA), high levels of tauP301L in the resulting animals can be regulated with tetracycline or its analog doxycycline (dox). For example, breeding to Camk2a-tTA mice (see Stock Nos. <a href="https://www.jax.org/strain/003010">003010</a> / <a href="https://www.jax.org/strain/007004">007004</a>) results in rT2 mice with conditional (inducible/reversible) tauP301L expression in the forebrain. rT2 mice express a 5 fold increase in levels of tauP301L protein as compared to rTg4510 mice, and do not exhibit the 20% loss of forebrain mass and gross forebrain atrophy found in 7 month old rTg4510. However, 13 and 15 month old rT2 mice develop severe forebrain mass loss and forebrain atrophy. The rT2 mice represent an alternative model of high tauP301L expression in the forebrain and may be useful in the study of Alzheimer's disease.

Use of MICE by companies or for-profit entities requires a license prior to shipping.

T2 mice allow Tet-Off/Tet-On expression of the pathogenic P301L mutant variant of human microtubule-associated protein tau (MAPT). The tauP301L construct is inserted downstream of Col1a1 and does not affect the expression of any endogenous genes. These mice may be useful in generating animals with conditional (inducible/reversible) tauP301L expression for studying the formation of neurofibrillary tangles associated with Alzheimer's disease, neurodegenerative tauopathies and frontotemporal dementia.

Typically mice are recovered in 12-16 weeks. Contact Customer Service to place an order or for more information.

<a target="_blank" href="https://www.jax.org/jax-mice-and-services/customer-support" rel="noreferrer">Contact Customer Service</a> for more information.