Cardiac cells expressing Ly6a (lymphocyte antigen 6 complex, locus A; also called Sca1) predominantly contribute to the cardiac vasculature, particularly in the endothelial cell expansion and remodeling that occurs early after birth or following myocardial infarction (MI). These Ly6aCre knock-in/knock-out mice express cre recombinase from the mouse Ly6a promoter in Ly6a-expressing cells, including cardiac vascular endothelial cells. Expression of Ly6a is eliminated by the targeted allele. Though present in the targeting construct, IRES-driven expression of nuclear localized EGFP is not observed. Heterozygotes and homozygotes are viable and fertile with no overt developmental or adult phenotype. Expression of a cre-dependent TdTomato reporter has been confirmed in the heart and kidney via immunohistochemistry. Crosses with cre-responsive R26-TdT reporter mice (see Stock No. <a href="https://www.jax.org/strain/007914"">007914</a>) enable permanent labeling and lineage tracing of cells that normally express Ly6a throughout development and postnatal growth, or after cardiac injury. Though rare cardiomyoctes are labeled, this may be the result of fusion events from lineage-traced leukocytes. Approximately 30% of total bone marrow cells are labeled (mostly mature hematopoietic cells).

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Ly6aCre knock-in/knock-out mice express cre recombinase and nuclear-localized EGFP from the mouse Ly6a (Sca1) promoter in Ly6a-expressing cells, including cardiac vascular endothelial cells.

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