Hip1r knock-out mice may be useful in studying gastric epithelial cell homeostasis, clathrin-coated pits and vesicular trafficking.
Theodora S Ross, University of Texas Southwestern Medical Center
The targeted Hip1r (huntingtin interacting protein 1 related) gene encodes a membrane protein and is found in clathrin-coated pits and is involved in vesciular trafficking. These mutant mice carry a knock-out allele of the Hip1r gene in which exons 2 through 8 have been replaced by a floxed neo cassette (in reverse orientation). Homozygotes are viable and fertile. No gene product (protein) is detected by western blot analysis of tissues from homozygotes (liver, spleen, pancreas, stomach, small intestine, large intestine, kidney, adrenal, bladder, testis, epidydimus, prostate, seminal vesicle, thymus, heart, lung, muscle, eye, brain). Northern blot analysis of brain tissue from homozygous mice does not detect wildtype mRNA.
A targeting vector designed by Dr. Theodora S. Ross (University of Michigan Medical School) containing a reverse-oriented floxed neo cassette was used to disrupt exons 2 through 8 of the Hip1r gene. The construct was electroporated into 129X1/SvJ-derived RW1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric males were bred to C57BL/6 female mice. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
|Allele Name||targeted mutation 1, Theodora S Ross|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Hip1r, huntingtin interacting protein 1 related|
|Strain of Origin||129X1/SvJ|
|Molecular Note||Exons 2-8 were replaced with a neomycin resistance gene in the opposite orientation of the endogenous gene. Northern blot demonstrates absence of wild-type RNA in mutant brains and Western blot confirms absence of protein in mutant tissues.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Hip1r KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #032596 in your Materials and Methods section.