B6.129X1-Slc2a8^tm1Thor/J

Stock number: 032491
Product name: glut8 loxP; glut8 flox
Research areas: Cell Biology Research, Neurobiology Research, Research Tools

Description

These glut8 loxP mutant mice possess loxP sites flanking exon 10 of the Slc2a8 gene. This strain may be useful for generating conditional mutations in applications related to hippocampal neurogenesis.

Detailed description

The targeted Slc2a8 gene encodes an intracellular glucose transporter. These mice possess loxP sites flanking exon 10 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 10 deleted in the cre-expressing tissues. Removal of the floxed sequence creates a null allele.

When bred to a strain with Cre recombinase expression in the germline, this mutant mouse strain may be useful in studies of hippocampal neurogenesis.

Development

A targeting vector was designed to insert a loxP site and an FRT site-flanked PGK-Neo selection cassette downstream of exon 10 and the polyadenylation site of the targeted gene, and to insert another loxP site upstream of exon 10. This construct was electroporated into 129X1/SvJ derived GS-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were bred to C57BL/6 to achieve germline transmission. The mice were then backcrossed to C57BL/6 for more than 5 generations. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

Allele

Symbol: Slc2a8^tm1Thor
Name: targeted mutation 1, Bernard Thorens
MGI accession ID: MGI:6202437
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed)
Marker symbol: Slc2a8
Marker name: solute carrier family 2, (facilitated glucose transporter), member 8
Marker synonyms: D2Ertd44e; GLUT8; GlutX1
Chromosome: 2
Strain of origin: 129X1/SvJ
Molecular note: A targeting vector is designed to insert a loxP site and an FRT site-flanked PGK-Neo selection cassette downstream of exon 10 and the polyadenylation site of the targeted gene, and to insert another loxP site upstream of exon 10.