Viperin- mice contain a neo cassette replacing exons 1-2 of the radical S-adenosyl methionine domain containing 2 (Rsad2) gene, abolishing gene expression. These exons encode 166 amino acids known to be critical for viperin's endoplasmic reticulum (ER) localization and antiviral activity. These mice may be useful when studying virus replication and pathology.
Peter Cresswell, Yale School of Medicine
Viperin- mice contain a neo cassette replacing exons 1-2 of the radical S-adenosyl methionine domain containing 2 (Rsad2) gene, abolishing gene expression. Viperin is an endoplasmic reticulum (ER)-associated antiviral protein which plays a major role in the cell antiviral state induced by type I and type II interferon. It can inhibit a wide range of DNA and RNA viruses, including human cytomegalovirus (HCMV), hepatitis C virus (HCV), west Nile virus (WNV), dengue virus, sindbis virus, influenza A virus, sendai virus, vesicular stomatitis virus (VSV), chikungunya virus (CHIKV), and human immunodeficiency virus (HIV-1). Exons 1-2 encode 166 amino acids known to be critical for viperin's ER localization and antiviral activity. Mice homozygous for this allele are viable and fertile. Splenic CD4+ T cells from these mice exhibit normal anti-T-cell receptor (TCR)-induced proliferation and IL-2 production, but produced significantly less T helper 2 (Th2) cytokines, including IL-4, IL-5, and IL-13, in association with impaired GATA3 activation, after stimulation with anti-CD3 antibody, which was not restored upon costimulation with anti-CD28.
The viperin- targeting vector was designed to replace exons 1-2 of the radical S-adenosyl methionine domain containing 2 (Rsad2) gene with a neomycin resistance (neo) cassette in reverse orientation to the gene. The construct was electroporated into 129/Sv-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric males were bred to C57BL/6J females to generate a colony of viperin-/- mice. These mice were backcrossed at least 7 generations to C57BL/6J mice (Stock No. 000664) prior to sending males to The Jackson Laboratory Repository. Upon arrival, sperm was cryopreserved. To generate our live colony, an aliquot of the frozen sperm was used to fertilize oocytes from C57BL/6J mice.
|Allele Name||targeted mutation 1, Keh-Chuang Chin|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Rsad2, radical S-adenosyl methionine domain containing 2|
|Strain of Origin||129/Sv|
|Molecular Note||Exon 1 and 2 were replaced with a neo cassette. The absence of transcript expression was confirmed by RT-PCR on splenic CD4+ T cell extracts. The absence of protein expression was confirmed by immunohistochemistry on the spleen and stimulated CD4+ T cells.|
When maintaining a live colony, homozygous mice may be bred together.
When using the viperin- mouse strain in a publication, please cite the originating article(s) and include JAX stock #032321 in your Materials and Methods section.