C57BL/6N-Gt(ROSA)26Sor^{tm1(CAG-Il11)Cook}/J

Stock number: 031928
Product name: Rosa26-Il11
Research areas: Cancer Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

Cre-mediated excision of a loxP-flanked STOP cassette enables conditional expression of mouse Il11 from a CAG promoter within the Gt(ROSA)26Sor gene. This strain has been useful in studies of cardiac fibrosis.

Detailed description

Upregulation of Il11 expression is the dominant transcriptional response to TGFβ1 (TGFB1, transforming growth factor, beta 1) exposure in fibroblasts associated with fibrotic cardiovascular disease. Il11 may provide a viable therapeutic target with fewer side effects than direct inhibition of TGFB1, a gene with pleiotropic roles.

In this targeted mutant strain, Cre-mediated excision of a loxP-flanked STOP cassette enables conditional expression of mouse Il11 from a CAG promoter within the Gt(ROSA)26Sor gene.

Expression of Il11 can be directed to fibroblasts through crosses with Tg(Col1a2-cre/ERT,-ALPP)7Cpd (see Stock No. 029567 or 029235) and tamoxifen-induced expression of cre recombinase.

Development

The mouse Il11 cDNA is expressed under the control of the ubiquitous cytomegalovirus (CMV) immediate early enhancer and the chicken β-actin (CAG) promoter. A loxP-flanked STOP cassette was introduced in between the CAG promoter and the Il11 insertion so that overexpression can be conditionally induced by Cre recombinase. The mutation was introduced into the Gt(ROSA)26Sor gene locus via homologous recombination in C57BL/6N-derived embryonic stem cells. This strain was maintained on a C57BL/6N genetic background by the donating laboratory.

Allele

Symbol: Gt(ROSA)26Sor^{tm1(CAG-Il11)Cook}
Name: targeted mutation 1, Stuart A Cook
MGI accession ID: MGI:6119573
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); Inserted expressed sequence
Synonyms: Rosa26-Il11
Marker symbol: Gt(ROSA)26Sor
Marker name: gene trap ROSA 26, Philippe Soriano
Marker synonyms: beta geo; Gtrgeo26; Gtrosa26; R26; ROSA26; SETD5-AS1; Thumpd3as1
Chromosome: 6
Strain of origin: C57BL/6N
Expressed genes: Il11,interleukin 11,mouse, laboratory
Molecular note: A construct containing mouse Il11 cDNA under the control of the ubiquitous cytomegalovirus immediate early enhancer and the chicken beta-actin promoter and a loxP-flanked STOP cassette between the promoter and the cDNA was inserted into the locus. Overexpression can be conditionally induced by Cre recombinase which removes the STOP cassette.