This Batf2 knockout strain exhibits decreased numbers of lung-resident CD103+ CD11b- DCs and CD103- CD11b- macrophages after infection by Toxoplasma gondi. They may be useful in applications related to the study of dendritic cell priming of T lymphocytes in responses to intracellular pathogens.
Wayne Yokoyama, Washington University Sch. Med., HHMI
A loxP-flanked and frt-flanked neo cassette replaces exons 1-2 of the basic leucine zipper transcription factor, ATF-like 2 (Batf2). BATF2 is a transcription factor which is involved in the development of the CD8α+ dendritic cells (DCs) that prime CD8. BATF2 is induced by LPS and IFN-γ in macrophages and CD103+ DC populations in response to infection. Mice that are homozygous for this allele are viable and fertile. These Batf2-/- mice exhibit decreased survival after decreased numbers of lung-resident CD103+ CD11b- DCs and CD103- CD11b- macrophages after infection by Toxoplasma gondi.
Of note, this allele is also available on a 129S6 coisogenetic background as Stock No. 019085.
A targeting vector was designed to replace exons 1-2 of the basic leucine zipper transcription factor, ATF-like 2 (Batf2) with, from 5' to 3', a loxP site, a frt site, a neomycin resistance (neo) cassette in reverse orientation to the gene, a second loxP site, and a second frt site. The construct was electroporated into 129S6/SvEvTac-derived EDJ22 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric males were bred to 129S6/SvEv females. A few of these mice were acquired by Dr. Wayne Yokoyama (Washington University School of Medicine) who bred them to CMV-cre mice on a C57BL/6 genetic background to remove the neo cassette. Mice were further backcrossed to C57BL/6NCrl mice to create this congenic strain. Cryopreserved sperm was sent to The Jackson Laboratory Repository. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6NJ (Stock No. 005304) oocytes.
|Allele Name||targeted mutation 1.1, Kenneth Murphy|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Batf2, basic leucine zipper transcription factor, ATF-like 2|
|Strain of Origin||129S6/SvEvTac|
|General Note||The version of this allele that contains the neomycin selection cassette, Batf2tm1Kmm, has been analyzed.|
|Molecular Note||The targeting vector is designed to replace exons 1-2 with, from 5' to 3', a loxP site, an FRT site, a neomycin resistance (neo) cassette in reverse orientation to the gene, a second loxP site, and a second FRT site. Cre-mediated recombination removed the neo cassette.|
When maintaining a live colony, homozygous mice may be bred together.
When using the Batf2 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #031903 in your Materials and Methods section.